机构：[1]Department of Dermatology, e First Affiliated Hospital of Yunnan Traditional Chinese Medicine University, No. 120 Guanghua Rd, Kunming, Yunnan 650021, China[2]College of Basic Medicine, Yunnan University of Traditional Chinese Medicine, No. 1076, Yuhua Rd, Kunming, Yunnan 650500, China[3]Department of Dermatology, Affiliated Calmette Hospital of Kunming Medical University and e First People’s Hospital of Kunming City, 504 Qingnian Rd, Kunming, Yunnan 650022, China[4]Department of Gerontology, e First Affiliated Hospital of Yunnan Traditional Chinese Medicine University, No. 120 Guanghua Rd, Kunming, Yunnan 650021, China
Background. Psoriasis is a common chronic inflammatory skin disease with multifactor etiology, characterized by abnormal proliferation and differentiation of keratinocytes. Huang-Lian Jie-Du decoction (HLJDD) is a traditional Chinese medicine prescription with good clinical curative effect on psoriasis. However, its therapeutic mechanisms are still unclear. Methods. The psoriasis model of SKH-1 nude mice was established by imiquimod-induced and HLJDD gavage was given. Hematoxylin and eosin staining were used to evaluate pathological morphologies, and immunohistochemistry was used to detect the expressions of Wnt1, beta-catenin, and c-Myc in psoriasis mice. Western blot was used to examine the expressions of Frizzled-2, LRP5/6, GSK-3 beta, APC, Axin2, TCF4, LEF1, cyclin D1, TBX3, EPHB2, and NOTUM enzyme. Results. In this study, HLJDD reduced skin erythema and lesions, decreased the thickness of epidermal and downregulated the expressions of Wnt1, beta-catenin, and c-Myc. Western blot results showed that HLJDD reduced the expressions of Wnt receptors Frizzled-2 and LRP5/6, and Wnt downstream target genes TCF4, LEF1, cyclin D1, TBX3, and EPHB2, while upregulated destruction complex proteins GSK-3 beta, APC, and Axin2. Conclusions. HLJDD can effectively treat psoriasis and inhibit the Wnt/beta-catenin signaling pathway at multiple stages.
基金：
National Natural Science
Foundation of China (81503479), Yunnan Provincial Science
and Technology Department-Applied Basic Research
Joint Special Funds of Yunnan University of Chinese
Medicine 2019FF002(-032), Innovation Team of Yunnan
Province-Traditional Chinese Medicine Prevention and
Treatment of Skin Diseases of .e First Affiliated Hospital of
Yunnan Traditional Chinese Medicine University
(2019HC001), Yunnan Academician Expert Workstation
(202105AF150035), and Dermatology Clinical Center of
Traditional Chinese Medicine in Yunnan Province.
第一作者机构：[1]Department of Dermatology, e First Affiliated Hospital of Yunnan Traditional Chinese Medicine University, No. 120 Guanghua Rd, Kunming, Yunnan 650021, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Xuesong,Luo Guangyun,Fu Lan,et al.Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/beta-Catenin Signaling Pathway in Psoriasis Mouse Model[J].EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.2022,2022:doi:10.1155/2022/3193572.
APA：
Yang, Xuesong,Luo, Guangyun,Fu, Lan,Huang, Hong,Wang, Lifen...&Ye, Jianzhou.(2022).Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/beta-Catenin Signaling Pathway in Psoriasis Mouse Model.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2022,
MLA：
Yang, Xuesong,et al."Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/beta-Catenin Signaling Pathway in Psoriasis Mouse Model".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022.(2022)
