机构：[1]The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, 650500, PR China.云南省第一人民医院[2]Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming, Yunnan, 650032, PR China.[3]Pharmaceutical College &[4]Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, Kunming, 650032, Yunnan, PR China.[4]The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650000, PR China.
Introduction. Osteoporosis (OP) is characterized by microstructural degeneration of bone tissue, low bone mass, bone fragility and even brittle fracture (osteoporotic fracture, OPF). OP and OPF are common and there are many disadvantages to the current medications for OP/OPF. Osteoking is a traditional Chinese medicine (TCM) originating from the Yi nationality (Yunnan, China) that has been used to treat bone diseases for decades.Hypothesis/Gap Statement. This study will reveal the changes in the intestinal microbiota of OP rats after 70 days of osteoking treatment.Method. With duplication of sham and OP rats, eight groups were established, with six rats in each group. The intestinal microbiotas were analysed by 16S rDNA sequencing.Results. The results showed that osteoking changed the intestinal microbiota of sham rats and OP rats. The mechanism by which osteoking improves OP is related to the functions of the intestinal microbiota. After 70 days of treatment with osteoking, the contents of Pseudonocardia, Pedomicrobium, Variovorax, Niastella and Actinosynnema were decreased in OP rats. The functions of the above intestinal microbiota related to iron metabolism affected calcifediol and 25(OH)D, and measuring these bone metabolic indicators is required for further study.Conclusion. Osteoking changes the intestinal microbiota to improve OP, and further study which reveals these intestinal microbiota and mechanism is needed.
基金：
This study was supported by the National Science Foundation of China united fund U1502227, Applied Basic Research Foundation of Yunnan Province
202101AU070258, Yunnan Provincial Department of Education Science Research Fund Project 2022J0208. The above institutions did not participate in
the design of the study, collection, analysis, interpretation of data, or in writing the manuscript.
第一作者机构：[1]The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, 650500, PR China.[2]Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming, Yunnan, 650032, PR China.[3]Pharmaceutical College &
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推荐引用方式(GB/T 7714)：
Sun Yan,Zhang Hui-Jie,Chen Ran,et al.16S rDNA analysis of osteoporotic rats treated with osteoking.[J].JOURNAL OF MEDICAL MICROBIOLOGY.2022,71(6):doi:10.1099/jmm.0.001552.
APA：
Sun Yan,Zhang Hui-Jie,Chen Ran,Lee Wen-Hui&Zhao Hong-Bin.(2022).16S rDNA analysis of osteoporotic rats treated with osteoking..JOURNAL OF MEDICAL MICROBIOLOGY,71,(6)
MLA：
Sun Yan,et al."16S rDNA analysis of osteoporotic rats treated with osteoking.".JOURNAL OF MEDICAL MICROBIOLOGY 71..6(2022)
