机构：[1]Medical School, Kunming University of Science and Technology, Kunming, Yunnan, China.[2]Department of Hepatobiliary and Pancreatic Surgery, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.外科片肝胆外科云南省第一人民医院
Cholangiocarcinoma (CCA) is a disease that includes a variety of epithelial neoplasms characterized by the differentiation of cholangiocytes. M2 polarization is imperative to the development of CCA cells. In this study, we investigated the influence of secreted protein acidic and rich in cysteine (SPARC) on M2 polarization and CCA cell growth. We found that the SPARC level was amplified in M2-polarized macrophages and TAMs. In addition, the downregulation of SPARC prevented the M2 polarization of macrophages. Silencing SPARC inhibited the M2 macrophage-mediated effects on the proliferation, migration, and angiogenesis of CCA cells. Additionally, SPARC knockdown blocked the M2 polarization of macrophages by inhibiting the PI3K/AKT signaling. Moreover, an activator of PI3K signaling repressed the effect of SPARC knockdown on the M2 macrophage-induced elevation of proliferation, migration, and angiogenesis in CCA cells. In conclusion, SPARC contributes to the M2 polarization of macrophages to promote proliferation, migration, and angiogenesis of CCA cells, which provides new insight into the treatment of CCA.
基金：
Acknowled gements: This research was supported by the Yunnan Provincial High-I evel Medical
Tec hnical Talents Training Project (H-2019038), Joint project of Yunnan Provinc ial Department of
sC 1e nce
and .
technology and Kunming
Medical University on applied bas ic
research (202101AY070001-233)，Scientific Researching Fund of First People's Hospital of Yunnan
Province (KHBS- 2020-019)，Yunnan Provincial High- level Medical Technical Talents Tra ining
Project (L-2019016), The National Natural Science Foundation of China No. 81960514)， The .
Young and M iddle- aged Academic and Technical Leaders in Yunnan Province/Reserve Talent
Training (Pro gramNo.202005AC160017), Open Project of Gastroenterology C linical Medic ine
Center No.202 1LCZXXF-XH02), The Project of Kunming Medical joint No.2019FE001-010).
第一作者机构：[1]Medical School, Kunming University of Science and Technology, Kunming, Yunnan, China.[2]Department of Hepatobiliary and Pancreatic Surgery, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Deng Shi-Kang,Jin Yun,Jin Yan,et al.SPARC induces M2 polarization of macrophages to promote proliferation, migration, and angiogenesis of cholangiocarcinoma cells[J].NEOPLASMA.2022,69(5):1101-1107.doi:10.4149/neo_2022_220324N333.
APA：
Deng Shi-Kang,Jin Yun,Jin Yan&Wang Jun-Feng.(2022).SPARC induces M2 polarization of macrophages to promote proliferation, migration, and angiogenesis of cholangiocarcinoma cells.NEOPLASMA,69,(5)
MLA：
Deng Shi-Kang,et al."SPARC induces M2 polarization of macrophages to promote proliferation, migration, and angiogenesis of cholangiocarcinoma cells".NEOPLASMA 69..5(2022):1101-1107
