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Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development

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机构： [1]Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA [2]Massachusetts General Hospital Center for Cancer Research, Charlestown, MA 02129, USA [3]Department of Reproductive Medicine, the First People’s Hospital of Yunnan Province, Kunming, China [4]Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm 171 77, Sweden [5]BRCF Bioinformatics Core, University of Michigan, Ann Arbor, MI 48109, USA [6]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China [7]Department of Medicine, Harvard Medical School, Boston, MA 02115, USA [8]Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA [9]Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA [10]Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA [11]Innere Medizin I, Klinikum rechts der Isar, Technical University Munich, Munich, Germany

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Despite its clinical and fundamental importance, our understanding of early human development remains limited. Stem cell-derived, embryo-like structures (or embryoids) allowing studies of early development without using natural embryos can potentially help fill the knowledge gap of human development. Herein, transcriptome at the single-cell level of a human embryoid model was profiled at different time points. Molecular maps of lineage diversifications from the pluripotent human epiblast toward the amniotic ectoderm, primitive streak/mesoderm, and primordial germ cells were constructed and compared with in vivo primate data. The comparative transcriptome analyses reveal a critical role of NODAL signaling in human mesoderm and primordial germ cell specification, which is further functionally validated. Through comparative transcriptome analyses and validations with human blastocysts and in vitro cultured cynomolgus embryos, we further proposed stringent criteria for distinguishing between human blastocyst trophectoderm and early amniotic ectoderm cells.Copyright © 2022 Elsevier Inc. All rights reserved.

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出版当年[2022]版：

大类 | 1 区

医学

小类 | 1 区细胞生物学 1 区细胞与组织工程

最新[2023]版：

大类 | 1 区

医学

小类 | 1 区细胞与组织工程 1 区细胞生物学

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出版当年[2021]版：

Q1 CELL & TISSUE ENGINEERING Q1 CELL BIOLOGY

最新[2023]版：

Q1 CELL & TISSUE ENGINEERING Q1 CELL BIOLOGY

影响因子： 19.8 最新[2023版] 22.6 最新五年平均 25.269 出版当年[2021版] 27.802 出版当年五年平均 24.633 出版前一年[2020版] 23.9 出版后一年[2022版]

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第一作者机构： [1]Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

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通讯机构： [1]Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA [8]Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA [9]Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

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