Eupatilin attenuates the senescence of nucleus pulposus cells and mitigates intervertebral disc degeneration via inhibition of the MAPK/NF-kappa B signaling pathway
Intervertebral disc degeneration (IDD) is the main cause of low back pain. An increasing number of studies have suggested that inflammatory response or the senescence of nucleus pulposus (NP) cells is strongly associated with the progress of IDD. Eupatilin, the main flavonoid extracted from Artemisia, was reported to be associated with the inhibition of the intracellular inflammatory response and the senescence of cells. However, the relationship between eupatilin and IDD is still unknown. In this study, we explored the role of eupatilin in tumor necrosis factor-alpha (TNF-alpha)-induced activation of inflammatory signaling pathways and NP cell senescence, in the anabolism and catabolism of NP cell extracellular matrix (ECM) and in the effect of the puncture-induced model of caudal IDD in the rat. In vitro, eupatilin significantly inhibited TNF-alpha-induced ECM degradation, downregulated the expression of related markers of NP cells (MMP3, MMP9, and MMP13), and upregulated the expression of SOX9 and COL2A1. Furthermore, eupatilin reduced TNF-alpha-induced cell senescence by inhibiting the expression of the senescence of NP cell-related markers (p21 and p53). Mechanistically, ECM degradation and cell senescence were reduced by eupatilin, which inhibited the activation of MAPK/NF-kappa B signaling pathways. Consistent with the in vitro data, eupatilin administration ameliorated the puncture-induced model of caudal IDD in the rat. In conclusion, eupatilin can inhibit the inflammatory response and the senescence of NP cells, which may be a novel treatment strategy for IDD.
基金：
National Natural Science Foundation of China; Shanghai Frontiers Science Center of Degeneration and Regeneration in Skeletal System; Scientific research fund project of Yunnan Provincial Department of Education; Scientific Research Project for High-level Talents of Yunnan University of Traditional Chinese Medicine; [82130073]; [BJ1-9000-22-4002]; [2022Y350]; [GCC202205]
第一作者机构：[1]Shanghai Ninth Peoples Hosp, Shanghai Key Lab Orthoped Implants, Shanghai, Peoples R China[2]Yunnan Univ Tradit Chinese Med, Clin Med Coll 2, Kunming, Yunnan, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Ninth Peoples Hosp, Shanghai Key Lab Orthoped Implants, Shanghai, Peoples R China[2]Yunnan Univ Tradit Chinese Med, Clin Med Coll 2, Kunming, Yunnan, Peoples R China[3]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Orthoped, Shanghai, Peoples R China[7]Yunnan St Johns Hosp, Kunming, Yunnan, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Huan,Yang Xiao,Rong Kewei,et al.Eupatilin attenuates the senescence of nucleus pulposus cells and mitigates intervertebral disc degeneration via inhibition of the MAPK/NF-kappa B signaling pathway[J].FRONTIERS IN PHARMACOLOGY.2022,13:doi:10.3389/fphar.2022.940475.
APA：
Yang, Huan,Yang, Xiao,Rong, Kewei,Liang, Jiarong,Wang, Zhengting...&Ye, Bin.(2022).Eupatilin attenuates the senescence of nucleus pulposus cells and mitigates intervertebral disc degeneration via inhibition of the MAPK/NF-kappa B signaling pathway.FRONTIERS IN PHARMACOLOGY,13,
MLA：
Yang, Huan,et al."Eupatilin attenuates the senescence of nucleus pulposus cells and mitigates intervertebral disc degeneration via inhibition of the MAPK/NF-kappa B signaling pathway".FRONTIERS IN PHARMACOLOGY 13.(2022)
医学