机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.苏州大学附属第二医院[2]Department of Laboratory Medicine, Sichuan Provincial People's Hospital, Chengdu, Sichuan, China.四川省人民医院[3]Center of Medical Laboratory, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.[4]Department of Laboratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.昆明医科大学附属第一医院[5]Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.中山大学附属第一医院[6]Laboratory Medicine Center, Aviation General Hospital, Beijing, China.[7]Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.重庆医科大学附属第一医院[8]Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA.[9]Department of Medical Affairs, Danaher Diagnostic Platform/Cepheid (China), New York, NY, USA.[10]Center for Discovery and Innovation, Hackensack-Meridian Health, Nutley, NJ 07110, USA.[11]Hackensack Meridian School of Medicine at Seton Hall University, Nutley, NJ 07110, USA.
Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was used to determine the genome sequence of 92 non-duplicated CR-Ent strains collected from multiple tertiary health centers. The precise species of Enterobacter strains were identified by average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH). Molecular features of high-risk CR-Ent sequence type (ST) lineages and carbapenemase-encoding plasmids were determined. The result revealed that the most common human-source CR-Ent species in China was E. xiangfangensis (66/92, 71.93%), and the proportion of carbapenemase-producing Enterobacter (CP-Ent) in CR-Ent was high (72/92, 78.26%) in comparison to other global regions. Furthermore, ST171 and ST116 E. xiangfangensis were the major lineages of CP-Ent strains, and ST171 E. xiangfangensis was more likely to cause infections in older patients. Genomic analysis also highlighted the likelihood of intra-hospital/inter-hospital clonal transmission of ST171 and ST116 E. xiangfangensis. In addition, the blaNDM-harboring IncX3-type plasmid was identified as the prevalent carbapenemase-encoding plasmid carried by CR-Ent strains, and was experimentally confirmed to be able to self-transfer with high frequency. This study detailed the genomic and clinical characteristics of CR-Ent in China in the form of multicenter for the first time. The high risk of carbapenemase-producing ST171 and ST116 E. xiangfangensis, and the blaNDM-harboring IncX3-type plasmid were detected and emphasized.
基金：
This study was supported by the Science Foundation of Jiangsu Province Health
Department (ZDB2020014); the Science Foundation of Suzhou Health Department
(LCZX202106); the Science and Technology Program of Suzhou (SZS201715); the
Discipline Construction Program of the Second Affiliated Hospital of Soochow
University (XKTJ-TD202001) and the Natural Science Fund of China (82002204)
第一作者机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.[*1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, China
推荐引用方式(GB/T 7714)：
Zhu Zhichen,Xie Xiaofang,Yu Hua,et al.Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: A multicenter genomic study[J].EMERGING MICROBES & INFECTIONS.2023,12(1):doi:10.1080/22221751.2022.2148562.
APA：
Zhu Zhichen,Xie Xiaofang,Yu Hua,Jia Wei,Shan Bin...&Du Hong.(2023).Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: A multicenter genomic study.EMERGING MICROBES & INFECTIONS,12,(1)
MLA：
Zhu Zhichen,et al."Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: A multicenter genomic study".EMERGING MICROBES & INFECTIONS 12..1(2023)
医学