机构：[1]Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shanxi, China.[2]Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan, China.
To determine the potential diagnostic and therapeutic targets of Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS).We selected the GSE11783, GSE57560 and GSE621 datasets from the GEO database and merged them. R software was used to screen differentially expressed genes (DEGs) between IC/BPS and normal bladder tissues. The "String" online tool is used to analyze DEGs interaction and functional protein enrichment. CIBERSORT online tool was used to analyze the infiltration of immune cells. In addition, we verified the function of BTK in IC/BPS at the clinical samples and cells level.Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins. At the same time, functional enrichment showed that they were significantly related to innate immunity. Immunoinfiltration showed that mast cell resting in IC/BPS was significantly higher. IHC staining of clinical samples showed that the mast cell markers Tryptase and BTK were highly expressed in IC/BPS tissues. At the cell level, knockdown of BTK inhibited proliferation, migration, invasion, and degranulation of mast cells.This study provides a new perspective for understanding the molecular mechanisms involved in IC/BPS and suggests that BTK may be a target for treating IC/BPS.
基金：
This study was supported by Grant No. 82160150
from the National Natural Science Foundation
of China, Grant No. 2019FE001(-149) and
No.202001AY070001-062 from Yunnan Provincial
Science and Technology Department / Kunming
Medical University Joint Project of Basic Research,
and Grant No. H-2017045 from Yunnan Medical
Science Specialist Training Project, and No.
202105AF150063 from Yunnan Provincial Science
and Technology Department
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|2 区医学
小类|3 区老年医学3 区细胞生物学
最新[2023]版：
大类|3 区医学
小类|3 区细胞生物学3 区老年医学
第一作者：
第一作者机构：[1]Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shanxi, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Guang,Yang Tong-Xin,Li Jiong-Ming,et al.Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome[J].Aging.2022,14(17):7052-7064.doi:10.18632/aging.204271.
APA：
Wang Guang,Yang Tong-Xin,Li Jiong-Ming,Huang Zi-Ye,Yang Wen-Bo...&He Da-Lin.(2022).Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome.Aging,14,(17)
MLA：
Wang Guang,et al."Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome".Aging 14..17(2022):7052-7064
