机构：[1]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Medical College, Soochow University, Suzhou, 215000, China. E-mail: mxiao@suda.edu.cn, mltang@suda.edu.cn[2]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China[3]Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, School of Life Science and Technology, Southeast University, Nanjing 210096, China[4]Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China[5]Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu 210008, China[6]Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, China.[7]Research Institution of Otorhinolaryngology, NanjingJiangsu 210008, P. R. China
Cochlear implantation is considered to be the best therapeutic method for profound sensorineural hearing loss, but insufficient numbers of functional spiral ganglion neurons hinder the clinical effects of cochlear implantation. Stem cell transplantation has the potential to provide novel strategies for spiral ganglion neuron regeneration after injury. However, some obstacles still need to be overcome, such as low survival and uncontrolled differentiation. Several novel technologies show promise for modulating neural stem cell behaviors to address these issues. Here, a device capable of electrical stimulation was designed by combining a cochlear implant with a graphene substrate. Neural stem cells (NSCs) were cultured on the graphene substrate and subjected to electrical stimulation transduced from sound waves detected by the cochlear implant. Cell behaviors were studied, and this device showed good biocompatibility for NSCs. More importantly, electric-acoustic stimulation with higher frequencies and amplitudes induced NSC death and apoptosis, and electric-acoustic stimulation could promote NSCs to proliferate and differentiate into neurons only when low-frequency stimulation was supplied. The present study provides experimental evidence for understanding the regulatory role of electric-acoustic stimulation on NSCs and highlights the potentials of the above-mentioned device in stem cell therapy for hearing loss treatment.
基金：
Major State Basic
Research Development Program of China (2017YFA0104303),
the National Natural Science Foundation of China (No.
81970883, 81622013, 81470692, 31500852), the Jiangsu Province
Natural Science Foundation (BK20181435, BK20200862),
Boehringer Ingelheim Pharma GmbH, the Yingdong Huo Education
Foundation, the Fundamental Research Funds for the
Central Universities (YG2005005).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|2 区工程技术
小类|3 区材料科学：生物材料
最新[2023]版：
大类|3 区医学
小类|3 区材料科学：生物材料
第一作者：
第一作者机构：[1]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Medical College, Soochow University, Suzhou, 215000, China. E-mail: mxiao@suda.edu.cn, mltang@suda.edu.cn[2]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China[3]Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, School of Life Science and Technology, Southeast University, Nanjing 210096, China
共同第一作者：
通讯作者：
通讯机构：[1]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Medical College, Soochow University, Suzhou, 215000, China. E-mail: mxiao@suda.edu.cn, mltang@suda.edu.cn[3]Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, School of Life Science and Technology, Southeast University, Nanjing 210096, China[4]Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China[6]Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, China.[7]Research Institution of Otorhinolaryngology, NanjingJiangsu 210008, P. R. China
