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Er-Xian decoction exhibits protective activity in a rat model of asthenospermia through a mechanism associated with DJ-1 protein upregulation

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机构: [1]Nanjing University of Chinese Medicine, Nanjing, China. [2]Department of Urology Surgery, Beijing University of Chinese Medicine, Third Affiliated Hospital, Beijing, China. [3]Department of Andrology, Nanchang Reproductive Hospital, Nanchang, China. [4]Yunnan University of Chinese Medicine, Kunming, China. [5]Department of Andrology, Yunnan Provincial Hospital of Traditional Chinese Medicine Hospital ,The First Affiliated Hospital, Yunnan University of Chinese Medicine, Kunming, China.
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Asthenozoospermia (AZS) remains a significant clinical problem of male factor infertility. Er-Xian decoction (EXD) is a traditional Chinese medicine with potent antioxidant activity to treat AZS. To investigate the protective effects of EXD on sperm motility and deglycase (DJ)-1 expression in AZS model rats. Sixty mature male Sprague-Dawley rats (200 - 250 g) were randomized into five equally sized groups, including ornidazole (ORN)-induced AZS model group, or L-carnitine (0.1 g/kg) treated group or EXD group (7.5, 15, or 30 g crude drug/kg). Oxidative stress was assessed by measuring superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px). DJ-1 expression in testis and epididymis tissue was measured via qRT-PCR, Western blotting, and immunofluorescence staining. Hematoxylin and eosin staining was used to gauge morphological changes of testis and epididymis. Sperm motility was significantly reduced the AZS model group, while increased in the low-, intermediate-, and high-dose EXD treatment groups by 45.51%, 49.43%, and 58.31%, respectively (P < 0.001), which with a similar increase of 57.21% being observed in the L-carnitine treatment group. Relative to the control group, oxidative stress indices were significantly altered in AZS model rats, which exhibited significant reductions in SOD and GSH-Px levels and significantly increased MDA levels (49.44 ± 1.38 U/ml, 14.02 ± 0.70 U/ml, and 26.37 ± 1.03 nmol/ml, respectively). After EXD treatment, oxidative stress indexes were significantly improved relative to those in these model rats, with high-dose EXD yielding more significant improvements in these oxidative stress indices relative to L-carnitine treatment. While AZS model rats exhibited morphological abnormalities, tissue disorder, and reduced cell counts in the testis and epididymis, these were reversed by EXD treatment in a dose-dependent manner. EXD treatment was also associated with a significant increase in DJ-1 protein expression in testis and epididymis tissue samples relative to the levels observed in AZS model rats. EXD is firstly reported could significantly improve sperm motility in AZS rats and is more effective at higher dosage, even better than L-carnitine. The protective effect of EXD on sperm motility is based on the DJ-1 expression.

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出版当年[2021]版:
大类 | 3 区 医学
小类 | 3 区 生理学
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 生理学
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第一作者机构: [1]Nanjing University of Chinese Medicine, Nanjing, China. [2]Department of Urology Surgery, Beijing University of Chinese Medicine, Third Affiliated Hospital, Beijing, China.
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通讯机构: [5]Department of Andrology, Yunnan Provincial Hospital of Traditional Chinese Medicine Hospital ,The First Affiliated Hospital, Yunnan University of Chinese Medicine, Kunming, China. [*1]Department of Andrology, Yunnan Provincial Hospital of Traditional Chinese Medicine Hospital ,The First Affiliated Hospital, Yunnan University of Chinese Medicine, No. 120 Guanghua Street, Kunming 650021, China.
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