机构：[1]Institute of Cardiovascular Disease of Integrated Traditional Chinese and Western Medicine, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, No. 528, Zhangheng Road, Shanghai 201203, China.[2]School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Shanghai 201203, China.[3]State Key Laboratory of Phytochemistry and Plant Resources in West China and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Nujiangexanthone A (NJXA), a bioactive component isolated from the leaves of Garcinia nujiangensis, has been reported to exhibit anti-inflammatory, antioxidant, and antitumor effects. Our previous work has shown that NJXA induced G0/1 arrest and apoptosis, thus suppressing cervical cancer cell growth. The present study provides new evidence that NJXA can induce cell death in HeLa cells by promoting mitophagy. We first identified that NJXA triggered GFP-LC3 and YFP-Parkin puncta accumulation, which are biomarkers of mitophagy. Moreover, NJXA degraded the mitochondrial membrane proteins Tom20 and Tim23 and mitochondrial fusion proteins MFN1 and MFN2, downregulated Parkin, and stabilized PINK1. Additionally, we revealed that NJXA induced lysosome degradation and colocalization of mitochondria and autophagosomes, which was attenuated by knocking down ATG7, the key regulator of mitophagy. Furthermore, since mitophagy is induced under starvation conditions, we detected the cytotoxic effect of NJXA in nutrient-deprived HeLa cells and observed better cytotoxicity. Taken together, our work contributes to the further clarification of the mechanism by which NJXA inhibits cervical cancer cell proliferation and provides evidence that NJXA has the potential to develop anticancer drugs.
基金：
This research was funded by the Key-Area Research and Development Program of Guangdong
Province, grant number 2020B1111110003; NSFC-Joint Foundation of Yunnan Province, grant
number U1902213; and special grants from the China Postdoctoral Science Foundation, grant number
2020TQ0198.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区化学
小类|3 区生化与分子生物学3 区化学综合
最新[2025]版：
大类|3 区化学
小类|3 区生化与分子生物学3 区化学：综合
第一作者：
第一作者机构：[1]Institute of Cardiovascular Disease of Integrated Traditional Chinese and Western Medicine, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, No. 528, Zhangheng Road, Shanghai 201203, China.[2]School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Shanghai 201203, China.
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Cardiovascular Disease of Integrated Traditional Chinese and Western Medicine, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, No. 528, Zhangheng Road, Shanghai 201203, China.[2]School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Shanghai 201203, China.
推荐引用方式(GB/T 7714)：
Feng Jiling,Mansouripour Anahitasadat,Xi Zhichao,et al.Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy[J].Molecules (Basel, Switzerland).2021,26(10):doi:10.3390/molecules26102858.
APA：
Feng Jiling,Mansouripour Anahitasadat,Xi Zhichao,Zhang Li,Xu Gang...&Xu Hongxi.(2021).Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.Molecules (Basel, Switzerland),26,(10)
MLA：
Feng Jiling,et al."Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy".Molecules (Basel, Switzerland) 26..10(2021)
