Elevated expression of mitochondrial transcription elongation factor (TEFM) predicts poor prognosis in low grade glioma-an analysis of the Cancer Genome Atlas (TCGA) dataset
机构：[1]College of Basic Medical Sciences, Dali University, Dali 671000, China.[2]Key Laboratory for Clinical Biochemistry Test of High Education in Yunnan Province, Dali University, Dali 671000, China.[3]Department of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 310053, China.[4]Department of Reproductive Medicine, The First Affiliated Hospital of Dali University, Dali 671000, China.[5]School of Life Sciences, Yunnan University, Kunming 650091, China.
Mitochondrial transcription elongation factor (TEFM) is a key molecule for mitochondrial DNA (mtDNA) replication-transcription switch. TEFM regulates both transcription elongation and RNA processing in mitochondria. However, the expression level and prognostic value of TEFM in low grade glioma (LGG) remain unclear. Therefore, in this study, we aimed to evaluate the clinical significance and the prognostic value of TEFM in LGG based on publicly available data.The relative mRNA expression level of TEFM in non-tumor brain tissues and LGG tissues were retrieved from Gene Expression Profiling Interactive Analysis (GEPIA). The RNA-Seq expression of TEFM and clinical information in LGG patients were collected from the updated the Cancer Genome Atlas (TCGA) database by using R3.6.1 software. Next, the relationship between the mRNA expression of TEFM and clinicopathological characteristics were analyzed. Kaplan-Meier survival curves of overall survival (OS) and disease-free survival (DFS) were implemented for the relationship between the mRNA expression of TEFM and the prognosis of LGG patients. A Cox regression model was performed for the multivariate analysis of the factors affected the prognosis of LGG patients. GEPIA online tool was used to analyze the correlation between TEFM gene expression level and other related mitochondrial regulatory genes in LGG. Finally, The Gene Set Enrichment Analysis (GSEA) was performed to identify cell processes and molecular signaling cascades affected by TEFM.GEPIA analysis showed that the mRNA expression levels of TEFM in LGG were significantly higher than that of non-tumor tissue. Moreover, the mRNA expression of TEFM is significantly correlated with age, World Health Organization (WHO) grade, pathological types, headache history and supratentorial location (P<0.05). Kaplan-Meier analysis showed that a high expression level of TEFM mRNA indicated a poor prognosis in OS rate (log-rank, P<0.01). Multivariate Cox regression analysis showed that age, WHO grade, pathological types and supratentorial location were the independent prognostic factors of LGG patients. The mRNA expression levels of TEFM gene were positively correlated with the TFAM, TFB1M, TFB2M, MTERF1-F4 and NRF1 gene (P<0.01, R>0), but negatively correlated with the POLRMT gene (P<0.01, R=-0.18) in LGG. The GSEA revealed that genes associated with the cell cycle, RNA degradation, spliceosome, and ubiquitin mediated proteolysis signaling pathway were remarkably enriched in higher-TEFM versus lower-TEFM tumors.Our findings disclosed that the expression of TEFM mRNA was significantly upregulated in human LGG tissues compared to non-tumor brain tissues. More importantly, the elevated expression of TEFM mRNA may potentially predict poor OS in LGG patients.2020 Translational Cancer Research. All rights reserved.
基金：
This study was financially supported by the National Nature Science Foundation of China (No. 31760331, 31601155, 81860531, 81660583, 81560458 and 81760507), the Yunnan Provincial Science and Technology Innovation Team (2011CI123), the Reserve Talents of Young and Middle-aged Academic and Technical Leaders of Yunnan Province (No. 2017HB077), the Top Young Talents of Ten Thousand Talents Plan of Yunnan Province (No. 2019), the Postgraduate Program of Scientific Research Fund of Yunnan Provincial Department of Education (No. 2020Y0542), and the Science and Technology Innovation Team of Dali University (ZKPY2019308 and ZKLX2019320).
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外文
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出版当年[2020]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]College of Basic Medical Sciences, Dali University, Dali 671000, China.[2]Key Laboratory for Clinical Biochemistry Test of High Education in Yunnan Province, Dali University, Dali 671000, China.
共同第一作者：
通讯作者：
通讯机构：[1]College of Basic Medical Sciences, Dali University, Dali 671000, China.[2]Key Laboratory for Clinical Biochemistry Test of High Education in Yunnan Province, Dali University, Dali 671000, China.[5]School of Life Sciences, Yunnan University, Kunming 650091, China.[*1]College of Basic Medical Sciences, Dali University, 02 Hongsheng Road, Dali 671000, China.[*2]School of Life Sciences, Yunnan University, 02 Cuihu Road, Kunming 650091, China
推荐引用方式(GB/T 7714)：
Li Sufen,Wang Weisi,Zi Jiaji,et al.Elevated expression of mitochondrial transcription elongation factor (TEFM) predicts poor prognosis in low grade glioma-an analysis of the Cancer Genome Atlas (TCGA) dataset[J].Translational cancer research.2020,9(5):3610-3622.doi:10.21037/tcr.2020.04.16.
APA：
Li Sufen,Wang Weisi,Zi Jiaji,Sun Meitao,Mei Wen...&Xiong Wei.(2020).Elevated expression of mitochondrial transcription elongation factor (TEFM) predicts poor prognosis in low grade glioma-an analysis of the Cancer Genome Atlas (TCGA) dataset.Translational cancer research,9,(5)
MLA：
Li Sufen,et al."Elevated expression of mitochondrial transcription elongation factor (TEFM) predicts poor prognosis in low grade glioma-an analysis of the Cancer Genome Atlas (TCGA) dataset".Translational cancer research 9..5(2020):3610-3622
