机构：[1]Yunnan Province Hospital of Infection Disease, Kunming 650301, Yunnan, China[2]Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, Yunnan, China[3]The Agricultural Technology Service Center of Qu Shui Town, Jiangcheng County, Yunnan 665907, China[4]Center for Systems Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Suzhou Institute of Systems Medicine, Suzhou 215123, China[5]Yuxi Center for Disease Control and Prevention, Yixu 653000, Yunnan, China
In December 2017, a squirrel (Callosciurus phayrei) died 2 days after capture in Kunming, and its intestinal tract, heart, liver, spleen, lung, and kidney were subjected to metagenomics analysis. Reassembly and verification by reverse transcription PCR of contigs generated by next-generation sequencing yielded a 5176-nt sequence, which was designated "squirrel bocaparvovirus" (SQBOV). Phylogenetic trees based on the aa sequences of NS1, NP1, and VP1 showed that SQBOV formed an independent branch in the bocaparvovirus phylogenetic tree. The amino acid sequence identity of the NS1 of SQBOV to those of other bocaparvoviruses was below the threshold of 85% that is used to demarcate species within the genus, indicating that it should be considered a member of a new bocaparvovirus species. To our knowledge, this is the first report of a bocaparvovirus in squirrels. Our findings will enable further studies of viral diversity in rodents and of the genetic diversity and host range of bocaparvoviruses.
基金：
This work was supported by Grants from the National Major
Research and Development Projects (Grant no. 2016YFD0500300),
National Natural Science Foundation of China (Grant nos. 31660714,
81960605 and 31460669), Basic Research Projects of Yunnan Province
(Grant 2019FA015), Scientific Research Fund Project of the Yunnan
Provincial Department of Education (Grant no. 2018JS253) and the
Yunnan Science and Technology Talents and Platform Plan (Grant no.
2018HB046).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区医学
小类|4 区病毒学
最新[2023]版：
大类|4 区医学
小类|4 区病毒学
第一作者：
第一作者机构：[1]Yunnan Province Hospital of Infection Disease, Kunming 650301, Yunnan, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Jiali,Li Nan,Li Zhao,et al.Identification of a novel bocaparvovirus in a wild squirrel in Kunming, Yunnan Province, China[J].Archives of virology.2020,165(6):1469-1474.doi:10.1007/s00705-020-04613-7.
APA：
Wang Jiali,Li Nan,Li Zhao,Liu Lin,He Yuwen...&Wang Jinglin.(2020).Identification of a novel bocaparvovirus in a wild squirrel in Kunming, Yunnan Province, China.Archives of virology,165,(6)
MLA：
Wang Jiali,et al."Identification of a novel bocaparvovirus in a wild squirrel in Kunming, Yunnan Province, China".Archives of virology 165..6(2020):1469-1474
