机构：[1]Department of Ophthalmology, Fourth Affiliated Hospital of Kunming Medical University (the Second People’s Hospital of Yunnan Province)[2]Yunnan Eye Institute[3]The Ocular Disease and Clinical Medicine Research Center of Yunnan Province[4]The Ocular Disease Clinical Medicine Center of Yunnan Province, Kunming, China
Retinal pigment epithelium (RPE) degenerative death is an evident hallmark of advanced age-related macular degeneration (AMD). The present study aims to evaluate the protective effects of S-allyl L-cysteine (SAC), a bioactive component from aged garlic extracts, on the oxidative stress-related apoptosis of RPE cells and to investigate the potential underlying mechanisms.Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining were performed to evaluate the effects of SAC on the hydroquinone-treated human ARPE19 cells. The Reactive Oxygen Species (ROS) production was measured by virtue of flow cytometry or determined under an inverted fluorescence microscope. Furthermore, the expression of antioxidant factor Nrf2, as well as downstream antioxidant genes, including NQO1, SOD1, SOD2, and HO1 was assessed in hydroquinone stimulated ARPE19 cells, in the presence or absence of SAC pretreatment.Hydroquinone incitement contributed to a marked decrease in cell viability, but enhanced cell apoptosis, whereas SAC addition did not cause significant alterations. When cells were pre-treated with SAC, cell proliferation was dramatically enhanced whereas apoptosis was mitigated, and the ROS generation induced by hydroquinone was also significantly suppressed, indicating a prominent function of SAC in preventing ARPE19 cells from oxidant-related apoptosis. The elevated expression levels of Nrf2 and other antioxidant genes driven by hydroquinone were downregulated by SAC addition.These data suggest that SAC can effectively attenuate hydroquinone-induced oxidative damage in human RPE cells. Our work is the first to demonstrate that SAC modulates oxidative stress-induced RPE apoptosis, thereby potentially proving new insights into the treatment of AMD.
基金：
This work was financially supported by the National Natural
Science Foundation of China (NSFC, Grant Number
81660167); the Science and Technology Project of Yunnan
Province (Grant Number 2017FB114); Key Laboratory of
Yunnan Province for the Prevention and Treatment of Ophthalmology
(2017DG008); Technical Research for the Prevention
and Treatment of Important Ocular Surface Diseases
(2018ZF009); Provincial Innovation Team for Cataract
and Ocular Fundus Disease, The Second People’s Hospital
of Yunnan Province (2017HC010); and Expert Workstation
of Yao Ke (2017IC064) and Graduate Innovation Fund of
Kunming Medical University (2019S197).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|3 区药学
最新[2023]版：
大类|4 区医学
小类|4 区药学
第一作者：
第一作者机构：[1]Department of Ophthalmology, Fourth Affiliated Hospital of Kunming Medical University (the Second People’s Hospital of Yunnan Province)[2]Yunnan Eye Institute[3]The Ocular Disease and Clinical Medicine Research Center of Yunnan Province[4]The Ocular Disease Clinical Medicine Center of Yunnan Province, Kunming, China
通讯作者：
通讯机构：[1]Department of Ophthalmology, Fourth Affiliated Hospital of Kunming Medical University (the Second People’s Hospital of Yunnan Province)[2]Yunnan Eye Institute[3]The Ocular Disease and Clinical Medicine Research Center of Yunnan Province[4]The Ocular Disease Clinical Medicine Center of Yunnan Province, Kunming, China
推荐引用方式(GB/T 7714)：
Sun Z-W,Chen C,Wang L,et al.S-allyl cysteine protects retinal pigment epithelium cells from hydroquinone-induced apoptosis through mitigating cellular response to oxidative stress[J].European review for medical and pharmacological sciences.2020,24(4):2120-2128.doi:10.26355/eurrev_202002_20392.
APA：
Sun Z-W,Chen C,Wang L,Li Y-D&Hu Z-L.(2020).S-allyl cysteine protects retinal pigment epithelium cells from hydroquinone-induced apoptosis through mitigating cellular response to oxidative stress.European review for medical and pharmacological sciences,24,(4)
MLA：
Sun Z-W,et al."S-allyl cysteine protects retinal pigment epithelium cells from hydroquinone-induced apoptosis through mitigating cellular response to oxidative stress".European review for medical and pharmacological sciences 24..4(2020):2120-2128
