机构：[1]Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming 650500 , China.[2]School of Life Science , Yunnan University , Kunming 650500 , China.[3]Department of Stomatology , Yan'an Hospital Affiliated to Kunming Medical University , Kunming 650031 , China.[4]Yunnan Key Laboratory of Panax notoginseng , Kunming University of Science and Technology , Kunming 650500 , China.
Oleanane-type saponins considered as the main medicinal ingredients in Panax japonicus are not found in Panax notoginseng. β-Amyrin synthase (βAS) was recognized as the first key enzyme in the biosynthetic branch of oleanane-type saponins. In this study, βAS gene from P. japonicus ( PjβAS) was transferred into P. notoginseng cells. Along with PjβAS expression in the transgenic cells, the expression levels of several key enzyme genes related to triterpenoid saponins biosynthesis and the content of P. notoginseng saponins were also increased. Two oleanane-type saponins, chikusetsusaponin IV and chikusetsusaponin IVa, contained in P. japonicus were first discovered in transgenic P. notoginseng cells. This study successfully constructed a biosynthetic pathway of oleanane-type saponins in P. notoginseng by introducing just one gene into the species. On the basis of this discovery and previous studies, the common biosynthetic pathway of triterpenoid saponins in Panax genus may be unified to some extent.
基金：
National Natural Science
Foundation of China (81560616, 31260070).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|1 区农林科学
小类|1 区农业综合2 区应用化学2 区食品科技
最新[2023]版：
大类|1 区农林科学
小类|1 区农业综合2 区应用化学2 区食品科技
第一作者：
第一作者机构：[1]Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming 650500 , China.
共同第一作者：
通讯作者：
通讯机构：[1]Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming 650500 , China.[4]Yunnan Key Laboratory of Panax notoginseng , Kunming University of Science and Technology , Kunming 650500 , China.
推荐引用方式(GB/T 7714)：
Zhang Xiang,Yu Yilin,Jiang Sen,et al.Oleanane-Type Saponins Biosynthesis in Panax notoginseng via Transformation of β-Amyrin Synthase Gene from Panax japonicus[J].Journal of agricultural and food chemistry.2019,67(7):1982-1989.doi:10.1021/acs.jafc.8b07183.
APA：
Zhang Xiang,Yu Yilin,Jiang Sen,Yu Hong,Xiang Yingying...&Ge Feng.(2019).Oleanane-Type Saponins Biosynthesis in Panax notoginseng via Transformation of β-Amyrin Synthase Gene from Panax japonicus.Journal of agricultural and food chemistry,67,(7)
MLA：
Zhang Xiang,et al."Oleanane-Type Saponins Biosynthesis in Panax notoginseng via Transformation of β-Amyrin Synthase Gene from Panax japonicus".Journal of agricultural and food chemistry 67..7(2019):1982-1989
农林科学