机构：[1]School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.[2]Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical medicine, Kunming Medical University, Kunming, 650500 Yunnan, China.[3]Yan'an Hospital of Kunming City, Kunming, 650051 Yunnan, China.[4]Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China.
Human umbilical cord mesenchymal stem cells (hUCMSCs) are superior to other sources of mesenchymal stem/stromal cells (MSCs), and they are used as a novel tool for cell-based cancer therapy. However, the mechanism underlying hUCMSC-induced cancer cell death is not clear. In the present study, we aimed to evaluate the effect of secreted factors of hUCMSCs on the breast cancer cell line MCF7 by exposing them to the conditioned medium (CM) of hUCMSCs. We evaluated the morphological changes, cell viability, cell cycle, apoptosis, DNA fragmentation, and interleukin-1β (IL-1β) secretion of CM-exposed MCF7 cells. The results showed that the secreted factors of hUCMSCs could cause MCF7 cell death by inducing pyroptosis. We also sequenced the total RNA, and the differentially expressed genes (DEGs) were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A total of 2597 (1822 upregulated and 775 downregulated) genes were identified and 14 pathways were significantly enriched. The results showed that the expression of the pyroptosis-related genes NLRP1 and CASP4 and the inflammation-related pathways changed significantly in MCF7 cells exposed to the CM. To the best of our knowledge, this study is the first to report that the secreted factors of hUCMSCs can cause MCF7 cell pyroptosis. Furthermore, it is the first to examine the global gene expression in MCF7 cells exposed to CM. These results will provide valuable information for further studies on the mechanism of MCF7 cell pyroptosis induced by the secreted factors of hUCMSCs. It will also help understand the effect of hUCMSCs on cell-based breast cancer therapy.
基金：
This work was supported by the
Major Science and Technology Projects of Yunnan Province
(grant number 2018ZF007-05), the National Natural Science
Foundation of China (grant number 81560043), the Foreign
Cooperation Project of Yunnan Province (grant number
2018IA045), and the Joint Special Funds for the Department
of Science and Technology of Yunnan Province-Kunming
Medical University (grant number 2017FE468 (-011)).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|2 区医学
小类|3 区细胞与组织工程
最新[2025]版：
大类|4 区医学
小类|4 区细胞与组织工程
第一作者：
第一作者机构：[1]School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.[2]Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical medicine, Kunming Medical University, Kunming, 650500 Yunnan, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Jiao Yang,Zhao Hongbo,Chen Guodong,et al.Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs[J].Stem cells international.2018,2018:5912194.doi:10.1155/2018/5912194.
APA：
Jiao Yang,Zhao Hongbo,Chen Guodong,Sang Xiongbo,Yang Luhan...&Zheng Bingrong.(2018).Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs.Stem cells international,2018,
MLA：
Jiao Yang,et al."Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs".Stem cells international 2018.(2018):5912194
