机构：[1]Second Department of Medical Oncology, The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China[2]2014 Grade of Queen Mary College of Medicine, Nanchang University, Nanchang 330006, China[3]Third Department of Internal Medicine, The Fifth People’s Hospital of Puyang, Puyang 457003, China[4]Department of Oncology, The First Affiliated Hospital of Dali University, Dali 671000, China[5]Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
Metastasis is the leading cause of breast cancer-related death. Chemokine (C-C motif) receptor 7 (CCR7) plays important roles in breast cancer metastasis. However, the role of CCR7 in triple-negative breast cancer (TNBC) has not been fully elucidated. In this study, we found that CCR7 is highly expressed in both TNBC cell lines and breast cancer tissues. CCR7 was knocked down by shRNA in 4T1 and MDA-MB-231, two TNBC cell lines, and we found that the depletion of CCR7 significantly decreased TNBC cell proliferation, migration and invasion in vitro. Furthermore, we confirmed that the knockdown of CCR7 reduced the distant metastasis of 4T1 cells in an orthotopic mouse model. Proteomic analysis in 4T1 cells indicated that several signaling pathways such as epithelial cell adhesion molecule might contribute to CCR7's function in breast cancer metastasis. Our results suggest that CCR7 promotes TNBC metastasis and may serve as a target for breast cancer diagnosis and treatment.
基金：
This work was supported by grants from the National Natural
Science Foundation of China (Nos. 81360393 and 81560432 to R.
Y., 81772847 and U1502222 to R.L. and U1602221 and 31771516
to C.C.).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|4 区生化与分子生物学4 区生物物理
最新[2023]版：
大类|2 区生物学
小类|2 区生物物理3 区生化与分子生物学
第一作者：
第一作者机构：[1]Second Department of Medical Oncology, The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wu Jiao,Li Lu,Liu Jianing,et al.CC chemokine receptor 7 promotes triple-negative breast cancer growth and metastasis[J].Acta Biochimica et Biophysica Sinica.2018,50(9):835-842.doi:10.1093/abbs/gmy077.
APA：
Wu Jiao,Li Lu,Liu Jianing,Wang Yang,Wang Zehua...&Yang Runxiang.(2018).CC chemokine receptor 7 promotes triple-negative breast cancer growth and metastasis.Acta Biochimica et Biophysica Sinica,50,(9)
MLA：
Wu Jiao,et al."CC chemokine receptor 7 promotes triple-negative breast cancer growth and metastasis".Acta Biochimica et Biophysica Sinica 50..9(2018):835-842
