机构：[1]School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.[2]Yunnan Institute for Food and Drug Control, Kunming 650011, China.[3]School of Pharmacy, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.[4]Central Laboratory, The No.1 Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021, China.
The present study was designed to assess the antiarthritic potential of ECF in collagen-induced arthritis (CIA) and explore its underlying mechanism. Methods. In vitro, lymphocyte proliferation assay was measured by CCK-8 kit. In vivo, the therapeutic potential of ECF on CIA was investigated; surface marker, Treg cell, and intracellular cytokines (IL-17A and IFN-��) were detected by flow cytometry. Th1 cell differentiation assay was performed, and mRNA expression in interferon-��-related signaling was examined by q-PCR analysis. Results. In vitro, ECF markedly inhibited the proliferation of splenocytes in response to ConA and anti-CD3. In vivo, ECF treatment reduced the severity of CIA, inhibited IFN-�� and IL-6 secretion, and decreased the proportion of CD11b+Gr-1+ splenic neutrophil. Meanwhile, ECF treatment significantly inhibited the IFN-�� expression in CD4+T cell without obviously influencing the development of Th17 cells and T regulatory cells. In vitro, ECF suppressed the differentiation of naive CD4+ T cells into Th1. Furthermore, ECF intensely blocked the transcriptional expression in interferon-��-related signaling, including IFN-��, T-bet, STAT1, and STAT4. Conclusion. Our results indicated that ECF exerted antiarthritic potential in collagen-induced arthritis by suppressing Th1 immune response and interferon-��-related signaling.
基金：
Natural Science Foundation of
China (Grant no. 81460624) and the Applied Basic Research
Program of Yunnan Province, China (Grant no. 2015FB199).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|3 区免疫学
最新[2023]版：
大类|3 区医学
小类|4 区免疫学
第一作者：
第一作者机构：[1]School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.[2]Yunnan Institute for Food and Drug Control, Kunming 650011, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Xu Shikui,Zuo Aixue,Guo Zengjun,et al.Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response[J].Journal of immunology research.2017,2017:7416792.doi:10.1155/2017/7416792.
APA：
Xu Shikui,Zuo Aixue,Guo Zengjun&Wan Chunping.(2017).Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response.Journal of immunology research,2017,
MLA：
Xu Shikui,et al."Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response".Journal of immunology research 2017.(2017):7416792
