机构：[1]Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China.[2]Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan.[3]Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan.[4]Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan.[5]Bangladesh Agricultural Research Institute, Gazipur, 1701, Bangladesh.
A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80-120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively. This is the first report of complete sequences of PCSV in mainland China. Phylogenetic analysis of all tospoviral proteins indicated that PCSV-14YV733 is closely related to members of the WSMoV serogroup.
基金：
National Natural Science Foundation of China (31371919), Yunnan
Provincial Key Lab of Agricultural Biotechnology (YBRI201501,
YBRI201505), Top Scientists Input Program of Yunnan
(2013HA028) and Yunling Scholar Program.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|4 区病毒学
最新[2023]版：
大类|4 区医学
小类|4 区病毒学
第一作者：
第一作者机构：[1]Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua Prefecture, Kunming, 650223, Yunnan, People's Republic of China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zheng Kuanyu,Chen Tsung-Chi,Yeh Shyi-Dong,et al.Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China[J].Archives of virology.2017,162(9):2809-2814.doi:10.1007/s00705-017-3402-5.
APA：
Zheng Kuanyu,Chen Tsung-Chi,Yeh Shyi-Dong,Rahman Md Siddiqur,Su Xiaoxia...&Dong Jiahong.(2017).Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China.Archives of virology,162,(9)
MLA：
Zheng Kuanyu,et al."Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China".Archives of virology 162..9(2017):2809-2814
