机构：[1]School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, China.[2]Department of Urology, The First Affiliated Hospital of Clinical Medicine of Guangdong Pharmaceutical University, Guangzhou, Guangdong, China.[3]Department of Clinical Pathology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China.[4]Deparement of Nuclear Medicine, People's Hospital of Yuxi City, Yuxi, Yunnan, China.[5]Department of Biomedical Engineering, The Johns University School of Medicine, Baltimore, USA.[6]Departments of Physiology and Functional Genomics, University of Florida, Gainesville, Florida, USA.
Ang-(1-7) inhibits lung cancer cell growth both in vitro and in vivo. However, the molecular mechanism of action is unclear and also the rapid degradation of Ang-(1-7) in vivo limits its clinical application. Here, we have demonstrated that Ang- (1-7) inhibits lung cancer cell growth by interrupting pre-replicative complex assembly and restrains epithelial-mesenchymal transition via Cdc6 inhibition. Furthermore, we constructed a mutant adeno-associated viral vector AAV8 (Y733F) that produced stable and high efficient Ang-(1-7) expression in a xenograft tumor model. The results show that AAV8-mediated Ang-(1-7) over-expression can remarkably suppress tumor growth in vivo by down-regulating Cdc6 and anti-angiogenesis. Ang-(1-7) over-expression via the AAV8 method may be a promising strategy for lung cancer treatment.
基金：
National Natural Science Foundation of China Grant 81072113 (H. Li), National 863 High Technique Development Project of China Grant 2012AA02A403 (H. Li), and in part from National Natural Science Foundation of China Grants 81272482 (J.Li), 81401920 (H.Du) as well as by awards the Doctoral Program of Higher Education of China Grant 20134433120020 (H. Du).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区肿瘤学3 区细胞生物学
最新[2025]版：
无
第一作者：
第一作者机构：[1]School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, China.
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推荐引用方式(GB/T 7714)：
Chen Xinglu,Chen Sansan,Pei Nana,et al.AAV-Mediated angiotensin 1-7 overexpression inhibits tumor growth of lung cancer in vitro and in vivo[J].Oncotarget.2017,8(1):354-363.doi:10.18632/oncotarget.13396.
APA：
Chen Xinglu,Chen Sansan,Pei Nana,Mao Yingying,Wang Shengyao...&Li Hongwei.(2017).AAV-Mediated angiotensin 1-7 overexpression inhibits tumor growth of lung cancer in vitro and in vivo.Oncotarget,8,(1)
MLA：
Chen Xinglu,et al."AAV-Mediated angiotensin 1-7 overexpression inhibits tumor growth of lung cancer in vitro and in vivo".Oncotarget 8..1(2017):354-363
