机构：[1]Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.[2]Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.[3]Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.[4]Department of Gynaecology and Obstetrics, Zhongxin Hospital, Qingdao 266000, Shandong, China.[5]Biological College, Southwest Forestry University, Kunming 650000, Yunnan, China.[6]Department of Gynaecology and Obstetrics, The First Clinical Medical College, Fujian Medical University, Fuzhou 350000, Fujian, China.[7]Department of Gynaecology and Obstetrics, Dongfang Hospital, Xiamen University, Fuzhou 350000, Fujian, China.
KIF2A, a member of the kinesin-13 family, has been reported to play a role in spindle assembly in mitosis. However, its function in mammalian meiosis remains unknown. In this research, we examined the expression, localization and function of KIF2A during mouse oocyte meiosis. KIF2A was expressed in some key stages in mouse oocyte meiosis. Immunofluorescent staining showed that KIF2A distributed in the germinal vesicle at the germinal vesicle stage and as the spindle assembling after meiosis resumption, KIF2A gradually accumulated to the entire spindle. The treatment of oocytes with taxol and nocodazole demonstrated that KIF2A was co-localized with α-tubulin. Depletion of KIF2A by specific short interfering (si) RNA injection resulted in abnormal spindle assembly, failure of spindle migration, misaligned chromosomes and asymmetric cell division. Meanwhile, SKA1 expression level was decreased and the TACC3 localization was disrupted. Moreover, depletion of KIF2A disrupted the actin cap formation, arrested oocytes at metaphase I with spindle assembly checkpoint protein BubR1 activated and finally reduced the rate of the first polar body extrusion. Our data indicate that KIF2A regulates the spindle assembly, asymmetric cytokinesis and the metaphase I-anaphase I transition in mouse oocyte.
基金：
National Natural Science Foundation of China (No.
31672248), the Fundamental Research Funds for the Central Universities (No. 20720150055) and Major State
Scientific Research Program of China (No. 2012CBA01303).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.[2]Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.[3]Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
通讯作者：
通讯机构：[1]Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.[3]Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
推荐引用方式(GB/T 7714)：
Chen Ming-Huang,Liu Yu,Wang Ya-Long,et al.KIF2A regulates the spindle assembly and the metaphase I-anaphase I transition in mouse oocyte[J].Scientific reports.2016,6:39337.doi:10.1038/srep39337.
APA：
Chen Ming-Huang,Liu Yu,Wang Ya-Long,Liu Rui,Xu Bai-Hui...&Wang Hai-Long.(2016).KIF2A regulates the spindle assembly and the metaphase I-anaphase I transition in mouse oocyte.Scientific reports,6,
MLA：
Chen Ming-Huang,et al."KIF2A regulates the spindle assembly and the metaphase I-anaphase I transition in mouse oocyte".Scientific reports 6.(2016):39337
