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Human Pre-gastrulation Embryo Culture in 3D Condition

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机构： [1]Department of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, China. [2]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China

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The development process of human embryo until blastocyst is well understood during the past 30 years, however, embryogenesis from blastocyst to pre-gastrulation was still remained a "black box". Limited by research materials and culture technologies, the "black box" is still unopened. We recently established an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537-542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.© 2023. Springer Science+Business Media, LLC.

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第一作者机构： [1]Department of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, China. [2]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China

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