The Comparison of Two Whole-Genome Amplification Approaches for Noninvasive Preimplantation Genetic Testing (ni-PGT) and the Application Scenario of ni-PGT during the Fresh Cycle
Recently, noninvasive preimplantation genetic testing (ni-PGT) using degenerate oligonucleotide primer PCR (DOP-PCR) and multiple annealing and looping-based amplification cycle (MALBAC)-based whole-genome amplification (WGA) methods has demonstrated predictable results in embryo testing. However, a considerable heterogeneity of results has been reported in numerous studies on these two WGA methods. Our aim was to evaluate the current WGA method for ni-PGT while further clarifying the applicable scenarios of ni-PGT in the fresh cycle. A total of 173 embryos were tested with trophectoderm biopsy and ni-PGT. In the whole preimplantation genetic testing, the clinical concordance rates of the detection results of DOP-PCR and MALBAC with the corresponding trophectoderm biopsy results were 64.12% (84/131) and 68.99% (89/129), respectively (P = 0.405). However, in the detection of abnormal embryos, the detection efficiency of ni-PGT is significantly improved [MALBAC: 96.55% versus 68.99% (P < 0.001); and DOP-PCR: 89.09% versus 64.12% (P < 0.001)]. In addition, the diagnostic efficiency of ni-PGT in low-quality blastocysts was significantly higher than that in high-quality blastocysts [MALBAC: 95.24% versus 51.85% (P = 0.001); and DOP-PCR: 91.30% versus 48.15% (P = 0.001)]. These results contribute to further understanding ni-PGT and to clarifying its application scenario in the fresh cycle.
基金：
Supported by the National Natural Science Foundation of China grants
31700798 (J.Y.), 32270981 (J.Y.), 82060282 (Yo.L.), and 82360305 (Z.W.);
High Level Project of Yunnan Province grants YNQR-QNRC-2018-126
(J.Y.) and XDYC-MY-2022-0055 (Z.W.); Yunnan Provincial Young and
Middle-aged Academic and Technical Leaders Reserve Talents Program
202305AC160022 (L.X.); Kunming Medical University Joint Program
202301AY070001-003 (J.Y.); Kunming University of Science and Technology
Joint Program KUST-KH2022025Y (J.Y.); and Open Project of Yunnan Provincial Reproductive and Obstetrics and Gynecology Clinical
Medicine Center (zx2019-01-01) grants 2020LCZXKF-SZ02 (J.Y.),
2021LCZXXF-SZ10 (X.W.), 2021LCZXXF-SZ01 (L.L.), 2020LCZXKFSZ03
(Yu.L.), 2020LCZXKF-SZ06 (N.L.), 2021LCZXXF-SZ09 (L.Z.),
2021LCZXXF-SZ03 (M.G.), 2021LCZXXF-SZ07 (L.T.), 2022LCZXKFSZ05
(Z.W.), 2022LCZXKF-SZ18 (M.T.), 2022LCZXKF-SZ15 (M.W.),
2022LCZXKF-SZ17 (Yu.L.), and 2022LCZXKF-SZ12 (J.Y.).
Hu Zhixin,Li Yonggang,Chen Kexin,et al.The Comparison of Two Whole-Genome Amplification Approaches for Noninvasive Preimplantation Genetic Testing (ni-PGT) and the Application Scenario of ni-PGT during the Fresh Cycle[J].JOURNAL OF MOLECULAR DIAGNOSTICS.2023,25(12):945-956.doi:10.1016/j.jmoldx.2023.09.005.
APA：
Hu, Zhixin,Li, Yonggang,Chen, Kexin,Li, Mingying,Tian, Mei...&Wu, Ze.(2023).The Comparison of Two Whole-Genome Amplification Approaches for Noninvasive Preimplantation Genetic Testing (ni-PGT) and the Application Scenario of ni-PGT during the Fresh Cycle.JOURNAL OF MOLECULAR DIAGNOSTICS,25,(12)
MLA：
Hu, Zhixin,et al."The Comparison of Two Whole-Genome Amplification Approaches for Noninvasive Preimplantation Genetic Testing (ni-PGT) and the Application Scenario of ni-PGT during the Fresh Cycle".JOURNAL OF MOLECULAR DIAGNOSTICS 25..12(2023):945-956
