BackgroundDry eye disease (DED) is an ocular illness caused by insufficient tear secretion or poor tear quality, and inflammation is a key factor in its pathogenesis. Previous studies have shown that miRNAs are important regulatory factors in DED.ObjectiveThe purpose of this study was to explore the potential mechanism by which miR-214-3p influenced the DED process by regulating the macrophage inflammatory response.MethodsWe induced THP-1 cells to differentiate into M0 macrophages with 100 ng/mL phorbol-12-myristate-13-acetate (PMA) and then added 15 ng/mL lipopolysaccharide (LPS) to induce inflammation. The expression of related genes and proteins was detected via RT-qPCR, Western blotting, ELISA and immunofluorescence staining; cell viability was measured using the CCK-8 assay; and flow cytometry was used to detect ROS levels.ResultsIn tear and serum samples from DED patients, the levels of miR-214-3p, IL-10, and Arg1 were decreased, and the levels of IL-6, TNF-alpha, IL-1 beta, and iNOS expression were increased. Moreover, the overexpression of miR-214-3p attenuated the effect of LPS and inhibited M1 polarization and inflammation in macrophages. Mechanistically, miR-214-3p inhibited macrophage ferroptosis by downregulating TFRC expression, thereby inhibiting macrophage M1 polarization and inflammation and alleviating the progression of DED.ConclusionsOur study indicated that the upregulation of miR-214-3p expression might be a new target for DED therapy.
基金：
Yunnan Basic Research
Program (202301AY070001-156), the National Natural Science and
Technology Foundation project (82360201), the Health Research Project of Kunming Health Commission (2020-07-02-112), and the Yunnan Province Young and Middle-aged Academic and Technical Leaders Reserve Talent Project (02405AC350103).
第一作者机构：[1]YanAn Hosp Kunming City, Dept Ophthalmol, Kunming 650051, Yunnan, Peoples R China
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推荐引用方式(GB/T 7714)：
Zhao Dandan,Ji Hao,Zhang Weijia,et al.miR-214-3p inhibits LPS-induced macrophage inflammation and attenuates the progression of dry eye syndrome by regulating ferroptosis in cells[J].GENES & GENOMICS.2024,doi:10.1007/s13258-024-01598-4.
APA：
Zhao, Dandan,Ji, Hao,Zhang, Weijia,He, Anni,Guo, Caizhe...&Liu, Yan.(2024).miR-214-3p inhibits LPS-induced macrophage inflammation and attenuates the progression of dry eye syndrome by regulating ferroptosis in cells.GENES & GENOMICS,,
MLA：
Zhao, Dandan,et al."miR-214-3p inhibits LPS-induced macrophage inflammation and attenuates the progression of dry eye syndrome by regulating ferroptosis in cells".GENES & GENOMICS .(2024)
