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CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma

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机构： [1]The first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, China. [2]The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan Province, Department of Otolaryngology, Kunming, China. [3]Kunming Medical University, School of Basic Medical Science, Department of Pathology and Pathophysiology, Kunming, Yunnan, China. [4]The Affiliated Hospital of Yunnan University, The Second People's Hospital of Yunnan Province, Department of Otolaryngology, Kunming, China. [5]The first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, China. Electronic address: hexg1018@163.com.

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This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC.The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analysis and survival curves were used to identify biomarkers associated with the prognosis of HSCC. In addition, the diagnostic value of the biomarkers and their relevance to the tumor microenvironment and immunotherapy were evaluated. A ceRNA network based on mRNAs, circRNAs, and miRNAs was established. Finally, qRT-PCR was used to evaluate the expression of biomarkers in HSCC and para-cancerous tissue samples.A network containing 90 miRNAs, 47 circRNAs, and 111 mRNAs was established via bioinformatic analysis. Three genes (NRG1, CCNG2, and CHSY1) were identified as prognostic and diagnostic biomarkers for HSCC. The low-CCNG2-expression and low-CHSY1-expression groups had higher survival rates, whereas the low-NRG1-expression group had a lower survival rate. The AUC values of the three biomarkers > 0.9, which indicated that the diagnostic value of all biomarkers was excellent. Moreover, CCNG2 and CHSY1 were associated with the severity of malignancy and the effectiveness of immunotherapy. Subsequently, a ceRNA network containing 3 mRNAs, 7 miRNAs, and 7 circRNAs was established. The results of qRT-PCR validated that the expression patterns of NRG1 and CHSY1 in clinical HSCC and para-cancerous samples were consistent with those observed in the GSE2379 dataset.In this study, we identified three diagnostic biomarkers for HSCC (NRG1, CCNG2, and CHSY1) and established their corresponding ceRNA network via bioinformatic analysis, providing novel insights into the screening and treatment of HSCC.Copyright © 2025 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier España S.L.U. All rights reserved.

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大类 | 4 区医学

小类 | 4 区耳鼻喉科学

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第一作者机构： [1]The first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, China.

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