机构：[1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 Hubei, P.R. China.[2]Department of Obstetrics and Gynecology/Reproductive Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071 Hubei, P.R. China.[3]Harvard Reproductive Endocrine Science Center and Reproductive Endocrine Unit of the Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.[4]Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 Hubei, P.R. China.[5]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming 650000 Yunnan, P.R. China昆明医科大学附属第一医院
Autophagy and apoptosis are two major modes of cell death. A balanced interplay between both is vital for phagocytic clearance of apoptotic testicular cells. Here, generating a SD rats model-treated with cadmium (Cd) to mimic environmental exposure on human, we show that autophagy and apoptosis present synchronous change trends in Cd-induced testicular injury/self-recovery. Further, the cross-talk of autophagy and apoptosis is investigated in four testicular cell lines (GC-1/GC-2/TM3/TM4 cells) respectively. Results reveal that Cd-exposure for five consecutive weeks induces reproductive toxicity in male rats. After one cycle of spermatogenesis within 8 weeks without Cd, toxic effects are ameliorated significantly. In vitro, we find that PI3K inhibitor 3-MA regulates apoptosis by inhibiting autophagy with mTOR-independent pathway in Cd-treated testicular cells. Conclusively, cross-talk between autophagy and apoptosis regulates testicular injury/recovery induced by Cd via PI3K with mTOR-independent pathway.
第一作者机构：[1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 Hubei, P.R. China.[2]Department of Obstetrics and Gynecology/Reproductive Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071 Hubei, P.R. China.[3]Harvard Reproductive Endocrine Science Center and Reproductive Endocrine Unit of the Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
通讯作者：
通讯机构：[1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 Hubei, P.R. China.[4]Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 Hubei, P.R. China.
推荐引用方式(GB/T 7714)：
Mei Wang,Xiao-fei Wang,Ya-min Li,et al.Cross-talk between autophagy and apoptosis regulates testicular injury/recovery induced by cadmium via PI3K with mTOR-independent pathway[J].CELL DEATH & DISEASE.2020,11(1):doi:10.1038/s41419-020-2246-1.
APA：
Mei Wang,Xiao-fei Wang,Ya-min Li,Na Chen,Yan Fan...&Ping Su.(2020).Cross-talk between autophagy and apoptosis regulates testicular injury/recovery induced by cadmium via PI3K with mTOR-independent pathway.CELL DEATH & DISEASE,11,(1)
MLA：
Mei Wang,et al."Cross-talk between autophagy and apoptosis regulates testicular injury/recovery induced by cadmium via PI3K with mTOR-independent pathway".CELL DEATH & DISEASE 11..1(2020)
生物学