机构：[1]Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091 Yunnan, P.R. China[2]School of Biomedical Sciences, HuaqiaoUniversity, 362021 Quanzhou, P.R. China[3]Biocontrol Engineering Research Center of Plant Disease & Pest, Kunming 650091 Yunnan, P.R. China[4]Biocontrol Engineering Research Center of Crop Disease & Pest, Kunming 650091 Yunnan, P.R. China[5]Biomedical Research Center, Calmette Hospital ofKunming Medical University (Kunming First People’s Hospital), Kunming 650011 Yunnan, P.R. China[6]Center for the Research and Technology of AgroEnvironmental and Biological Sciences (CITAB)/Department of Biology and Environment, Universidade de Trás-os-Montes e Alto Douro (UTAD), Apartado1013, 5001-801 Vila Real, Portugal[7]Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de Lisboa, 2780-157Oeiras, Portugal
The toxic kernel cake of Jatropha curcas (KCakeJ) is an emerging health and environmental concern. Although phorbol esters are widely recognized as the major toxin of KCakeJ, convincing evidence is absent. Here, we show that rather than phorbol esters an isomeric mixture of 11-hydroxy-9E-octadecenoic acid, 12-hydroxy-10E-octadecenoic acid and 12-hydroxy-10Z-octadecenoic acid (hydroxy-octadecenoic acids, molecular formula C18H34O3) is the major toxic component. The toxicities of hydroxy-octadecenoic acids on experimental animals, e.g. acute lethality, causing inflammation, pulmonary hemorrhage and thrombi, allergies, diarrhea and abortion, are consistent with those on human/animals caused by Jatropha seed and/or KCakeJ. The hydroxyl group and the double bond are essential for hydroxy-octadecenoic acids' toxicity. The main pathway of the toxicity mechanism includes down-regulating UCP3 gene expression, promoting ROS production, thus activating CD62P expression (platelet activation) and mast cell degranulation. The identification of the major toxin of KCakeJ lays a foundation for establishing an environmentally friendly Jatropha biofuel industry.
基金：
Natural Science Foundation of ChinaNational Natural Science Foundation of China [21366033, 21772047]; South and Southeast Asia Cooperation Base on Microbiological Resource Prevention and Utilization [2018IA100]; Portugal 2020 under the Operational Competitiveness and Internationalization Programme [006958, POCI-01-0145-FEDER-006958]; EU's European Regional Development FundEuropean Union (EU); Portugal national funds FCT-Portuguese Foundation for Science and Technology [POCI-01-0145-FEDER-006958, FCOMP-01-0124-0124-FEDER-022692, UID/AGR/04033/2019]; Jaime Cardoso e Freire LDA, Portugal
第一作者机构：[1]Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091 Yunnan, P.R. China
共同第一作者：
通讯作者：
通讯机构：[6]Center for the Research and Technology of AgroEnvironmental and Biological Sciences (CITAB)/Department of Biology and Environment, Universidade de Trás-os-Montes e Alto Douro (UTAD), Apartado1013, 5001-801 Vila Real, Portugal[7]Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de Lisboa, 2780-157Oeiras, Portugal
推荐引用方式(GB/T 7714)：
Wang Xing-Hong,Liu Jie-Qing,Chen Suiyun,et al.Hydroxy-octadecenoic acids instead of phorbol esters are responsible for the Jatropha curcas kernel cake's toxicity[J].COMMUNICATIONS BIOLOGY.2020,3(1):doi:10.1038/s42003-020-0919-z.
APA：
Wang, Xing-Hong,Liu, Jie-Qing,Chen, Suiyun,Yin, Yanfeng,Liu, Yan&Zhang, Changhe.(2020).Hydroxy-octadecenoic acids instead of phorbol esters are responsible for the Jatropha curcas kernel cake's toxicity.COMMUNICATIONS BIOLOGY,3,(1)
MLA：
Wang, Xing-Hong,et al."Hydroxy-octadecenoic acids instead of phorbol esters are responsible for the Jatropha curcas kernel cake's toxicity".COMMUNICATIONS BIOLOGY 3..1(2020)
生物学