机构：[1]Department of Rheumatology, the First People’s Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Scienceand Technology, Kunming 650032, Yunnan, China内科片风湿免疫科云南省第一人民医院[2]Department of Orthopaedics, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China昆明医科大学附属第一医院
Abstract—The miRNA miR-124 has been reported to be a promising target for the repair of spinal cord injury (SCI),
which is a devastating neurological condition. This study aimed to investigate the underlying molecular mechanisms
of miR-124-mediated SCI repair. We established miR-124 SCI model rats and further treated them with
agomiR-124 for 14 days. After that, their spinal cords were sectioned, and levels of NeuN, GFAP, and NF-200 were
measured via immunofluorescence or via immunohistochemistry. In addition, the spinal dorsal horns were collected
for sequencing of total RNA. Differentially expressed (DE) mRNAs were then profiled and a number of these
were further verified with qPCR. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes
(KEGG) pathway analysis were performed to predict the potential functions of the DE mRNAs. AgomiR-124 was
found to significantly inhibit the decrease of neurons and the activation of astrocytes, while promoting NF-200
expression in the dorsal horn. At fourteen days after agomiR-124 treatment, a total of 85 mRNAs were upregulated
and 80 mRNAs were downregulated. We focused our analysis of the DE mRNAs on the top 20 most DE mRNAs,
and found four upregulated genes (Nploc4, Yme1l1, LOC103693564, and Aspa) and four downregulated genes
(Epb41l2, LOC100911685, LOC100910833, and Smarcc1), which are likely to be of interest to SCI researchers. In
addition, we noted that Tal1 is a potential target gene of miR-124, and that a low level of this gene promoted
the proliferation of neuronal precursor cells and inhibited their differentiation. In conclusion, miR-124 was able
to mediate SCI repair by altering the expression of various mRNAs in rats. The miR-124/Tal1 axis may participate
in the treatment of SCI by agomiR-124 by repopulating neural stem cells. 2020 Published by Elsevier Ltd on behalf of
IBRO.
基金：
National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China [81660215, 81860093]; Associated Project of Yunnan Province Science & Technology Department and Kunming Medical University Basic Research for Application [2019FE001(-207)]
第一作者机构：[1]Department of Rheumatology, the First People’s Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Scienceand Technology, Kunming 650032, Yunnan, China
通讯作者：
通讯机构：[2]Department of Orthopaedics, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China[*1]Department of Orthopaedics, the First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming 650032, Yunnan, China
推荐引用方式(GB/T 7714)：
Wang Jing,Li Haotian,Chen Lingqiang,et al.mRNA Profiling for miR-124-mediated Repair in Spinal Cord Injury[J].NEUROSCIENCE.2020,438:158-168.doi:10.1016/j.neuroscience.2020.05.013.
APA：
Wang, Jing,Li, Haotian,Chen, Lingqiang,Dong, Junjie,Yang, Jin...&Zhao, Xueling.(2020).mRNA Profiling for miR-124-mediated Repair in Spinal Cord Injury.NEUROSCIENCE,438,
MLA：
Wang, Jing,et al."mRNA Profiling for miR-124-mediated Repair in Spinal Cord Injury".NEUROSCIENCE 438.(2020):158-168
