机构：[1]Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University,Tumor Hospital of Yunnan Province, Kunming,[2]Department of Medical Oncology, The First AffiliatedHospital of Kunming Medical University, Kunming,[3]Department of Thoracic Surgery, The ThirdAffiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming,[4]TheThird Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming,China
Background/Aims: This study aimed to investigate the potential roles of miR-424 expression in non-small cell lung cancer (NSCLC) metastasis and growth and its underlying mechanism. Methods: The expression of miR-424 in two NSCLC cell lines (A549 and H1975) was altered by transfection with miR-424 mimic and inhibitor. Effects of miR-424 overexpression and suppression on cells migration, invasion and colony formation were analyzed. Target genes for miR-424 were predicted using bioinformatics method and then verified using luciferase assay. Effects of miR-424 expression on cell migration, invasion and proliferation were reanalyzed on the condition of TNFAIP1 was silenced. Moreover, TNFAIP1 silencing and miR-424 modified A549 cells were subcutaneous injected into node BALB/c mice to confirm the regulation of miR-424 on TNFAIP1 in regulating tumor growth. Results: Compared with the control, miR-424 overexpression significantly increased the migrated and invaded cells, as well as the proliferated colonies. TNFAIP1 was a predicted target gene for miR-424, and was negatively regulated by miR-424. TNFAIP1 silence significantly increased the migrated and invaded cells compared to that in control, while these increases were abolished by miR-424 suppression. Animal experiment further evidenced miR-424 affected tumor growth by regulating TNFAIP1. Conclusions: These data demonstrate that miR-424 may be a contributor for NSCLC progression and metastasis through involving in cell migration, invasion and proliferation via inhibiting TNFAIP1. This study may provide theoretical basis for miR-424 in NSCLC target therapeutic treatment. (C) 2017 The Author(s) Published by S. Karger AG, Basel
基金：
National Science Foundation of Yunnan Province [2011FB206]
第一作者机构：[1]Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University,Tumor Hospital of Yunnan Province, Kunming,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University,Tumor Hospital of Yunnan Province, Kunming,[*1]Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, No.519, Kunzhou Road, Kunming 650018, (China)
推荐引用方式(GB/T 7714)：
Zhang Ming,Gao Chang'e,Yang Yi,et al.MiR-424 Promotes Non-Small Cell Lung Cancer Progression and Metastasis through Regulating the Tumor Suppressor Gene TNFAIP1[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2017,42(1):211-221.doi:10.1159/000477314.
APA：
Zhang, Ming,Gao, Chang'e,Yang, Yi,Li, Gaofeng,Dong, Jian...&Li, Wenhui.(2017).MiR-424 Promotes Non-Small Cell Lung Cancer Progression and Metastasis through Regulating the Tumor Suppressor Gene TNFAIP1.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,42,(1)
MLA：
Zhang, Ming,et al."MiR-424 Promotes Non-Small Cell Lung Cancer Progression and Metastasis through Regulating the Tumor Suppressor Gene TNFAIP1".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 42..1(2017):211-221
