机构：[1]Department of Hepatobiliary Surgery, the First People’s Hospital of Yunnan Province, Kunhua Hospital Affiliated to Kunming University of Science and Technology, Kunming 650031, P.R. China外科片肝胆外科云南省第一人民医院血管外科[2]Department of Nephrology, the First People’s Hospital of Yunnan Province, Kunhua Hospital Affiliated to Kunming University of Science and Technology, Kunming 650031, P.R. China内科片肾内科云南省第一人民医院[3]Department of Nutrition, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, P.R. China昆明医科大学附属第一医院[4]Department of Geriatric Cardiology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, P.R. China昆明医科大学附属第一医院[5]Department of Nephrology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032,P.R. China昆明医科大学附属第一医院
This study aims to explore the effect of PKC- gene silencing on the occurrence of ultrafiltration failure (UFF) in peritoneal dialysis (PD) rats. Forty-eight male SD rats were collected to establish 5/6 renal resection uremic and uremic PD rats models. Rats were assigned into control, sham operation, uremia, PD-2W (peritoneal dialysis for 2 weeks), PD-4W (peritoneal dialysis for 4 weeks), negative control (NC) (peritoneal dialysis for 4 weeks, and injected 0.1mg/kg blank plasmid into abdominal cavity) and PKC- siRNA (peritoneal dialysis for 4 weeks, and injected 0.1mg/kg PKC- siRNA into abdominal cavity) groups. CD34 staining was performed to determine microvessel density (MVD) for peritoneal tissues. The mRNA and protein expression of PKC- in peritoneal tissue were detected by qRT-PCR and Western blot. Compared with the control group, MVD, the mRNA and protein expression of PKC- were significantly increased in rats of the uremia, PD-2W, PD-4W, NC, and PKC- siRNA groups. Compared with the uremia group, MVD, the mRNA and protein expression of PKC- were increased, the changes observed in the PD-4W and NC groups were better obvious than in the PD-2W group. In comparison with the PD-4W and NC groups, MVD, the mRNA and protein expression of PKC- in rats were decreased in the PKC- siRNA group. PKC- gene has a high expression in uremic PD rats, and PKC- gene silencing is able to increase UF while decrease MVD and glucose transport in peritoneal tissues thus reversing UFF in PD rats. J. Cell. Biochem. 118: 4607-4616, 2017. (c) 2017 Wiley Periodicals, Inc.
第一作者机构：[1]Department of Hepatobiliary Surgery, the First People’s Hospital of Yunnan Province, Kunhua Hospital Affiliated to Kunming University of Science and Technology, Kunming 650031, P.R. China
通讯作者：
通讯机构：[5]Department of Nephrology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032,P.R. China[*1]Department of Nephrology, the First Affiliated Hospital of KunmingMedical University, No.295, Xichang Road, Kunming 650032, Yunnan Province,P.R. China
推荐引用方式(GB/T 7714)：
Sun Zhi-Wei,Wang Jian,Weng Min,et al.Role of Small Interfering RNA Silencing Protein Kinase C- Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats[J].JOURNAL OF CELLULAR BIOCHEMISTRY.2017,118(12):4607-4616.doi:10.1002/jcb.26125.
APA：
Sun, Zhi-Wei,Wang, Jian,Weng, Min,Tang, Jian-Zhong,Wang, Jun-Feng...&Yuan, Hong-Ling.(2017).Role of Small Interfering RNA Silencing Protein Kinase C- Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats.JOURNAL OF CELLULAR BIOCHEMISTRY,118,(12)
MLA：
Sun, Zhi-Wei,et al."Role of Small Interfering RNA Silencing Protein Kinase C- Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats".JOURNAL OF CELLULAR BIOCHEMISTRY 118..12(2017):4607-4616
