机构：[1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Instituteof digestive disease, Kunming 650032, China昆明医科大学附属第一医院[2]Department of General Surgery, The First Affiliated Hospital ofKunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China昆明医科大学附属第一医院
The transmembrane receptor guanylate cyclase-C (GC-C) signaling pathway has been implicated in several gastrointestinal disorders. Activation of GC-C via guanylin (Gn) and uroguanylin (Ugn) regulates intestinal fluid and electrolyte homeostasis. However, how it regulates the pathogenesis of inflammatory bowel disease (IBD) is still unclear. Here, we investigated the activation of GC-C signaling in ulcerative colitis (UC) of different clinical severities. A total of 60 UC patients and 20 normal controls were recruited. Evaluation of the UC disease activity index (DAI) was performed using a modified Mayo scoring system. The expression of GC-C, Gn and Ugn in the colonic mucosa was measured by quantitative real-time PCR and Western blot. We found that the UC patients had significantly lower expression of GC-C, Gn and Ugn than the controls. Furthermore, there were significant differences for GC-C, Gn and Ugn expression for the UC groups of Grade 1, 2 and 3, and their expression levels were reduced with increases in their DAI. Taken together, our results demonstrate that GC-C, Gn and Ugn are downregulated in UC, and this downregulation is more significant with aggravation of the clinical condition. Therefore, the GC-C signaling pathway may be implicated in the progression of UC.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81260074, 81160055]; Medical academic leader of Yunnan Provincial Bureau of Health [D-201215]; Social development of science and technology projects of Yunnan Province [2013CA021]; Foundation of Yunnan Institute of Digestive Disease [2014NS123]
第一作者机构：[1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Instituteof digestive disease, Kunming 650032, China
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推荐引用方式(GB/T 7714)：
Lan Danfeng,Niu Junkun,Miao Jiarong,et al.Expression of guanylate cyclase-C, guanylin, and uroguanylin is downregulated proportionally to the ulcerative colitis disease activity index[J].SCIENTIFIC REPORTS.2016,6:doi:10.1038/srep25034.
APA：
Lan, Danfeng,Niu, Junkun,Miao, Jiarong,Dong, Xiangqian,Wang, Hong...&Miao, Yinglei.(2016).Expression of guanylate cyclase-C, guanylin, and uroguanylin is downregulated proportionally to the ulcerative colitis disease activity index.SCIENTIFIC REPORTS,6,
MLA：
Lan, Danfeng,et al."Expression of guanylate cyclase-C, guanylin, and uroguanylin is downregulated proportionally to the ulcerative colitis disease activity index".SCIENTIFIC REPORTS 6.(2016)
