机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China[2]Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, China[3]Yuxi City Center for Disease Control and Prevention, Yuxi, Yunnan, China[4]Department of Dermatology, the First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, China.昆明医科大学附属第一医院
Leprosy is a chronic infectious and neurological disease that is caused by infection of Mycobacterium leprae (M. leprae). A recent genome-wide association study indicated a suggestive association of LRRK2 genetic variant rs1873613 with leprosy in Chinese population. To validate this association and further identify potential causal variants of LRRK2 with leprosy, we genotyped 13 LRRK2 variants in 548 leprosy patients and 1078 healthy individuals from Yunnan Province and (re-)analyzed 3225 Han Chinese across China. Variants rs1427267, rs3761863, rs1873613, rs732374 and rs7298930 were significantly associated with leprosy per se and/or paucibacillary leprosy (PB). Haplotype A-G-A-C-A was significantly associated with leprosy per se (P = 0.018) and PB (P = 0.020). Overexpression of the protective allele (Thr2397) of rs3761863 in HEK293 cells led to a significantly increased nuclear factor of activated T-cells' activity compared with allele Met2397 after lipopolysaccharides stimulation. Allele Thr2397 could attenuate 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced autophagic activity in U251 cells. These data suggest that the protective effect of LRRK2 variant p.M2397T on leprosy might be mediated by increasing immune response and decreasing neurotoxicity after M. leprae loading. Our findings confirm that LRRK2 is a susceptible gene to leprosy in Han Chinese population.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31271346, 30925021]; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2011CB910902]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31271346, 30925021]; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2011CB910902]
第一作者机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China
通讯作者：
通讯机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China[*1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, East Jiaochang Road No. 32, Kunming, Yunnan 650223, China.
推荐引用方式(GB/T 7714)：
Wang D.,Xu L.,Lv L.,et al.Association of the LRRK2 genetic polymorphisms with leprosy in Han Chinese from Southwest China[J].GENES AND IMMUNITY.2015,16(2):112-119.doi:10.1038/gene.2014.72.
APA：
Wang, D.,Xu, L.,Lv, L.,Su, L-Y,Fan, Y....&Yao, Y-G.(2015).Association of the LRRK2 genetic polymorphisms with leprosy in Han Chinese from Southwest China.GENES AND IMMUNITY,16,(2)
MLA：
Wang, D.,et al."Association of the LRRK2 genetic polymorphisms with leprosy in Han Chinese from Southwest China".GENES AND IMMUNITY 16..2(2015):112-119
