机构：[1]Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.重庆医科大学附属第一医院[2]Department of General Surgery, The First People’s Hospital of Yunnan Province, Kunming, China.云南省第一人民医院[3]Experimental Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China重庆医科大学附属第一医院
Epidermal growth factor receptor (EGFR) promotes proliferation of cancer cells. Dominant negative EGFR (DNEGFR) can block EGFR signal pathway by competing with endogenous EGFR for ligands. However, whether EGFR is overexpressed in gastric cancer and whether DNEGFR contributes to the inhibition of gastric cancer growth are not known. In this study, with the methods of immunohistochemistry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, flow cytometry, Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick-end labeling assay and western blotting; we demonstrate that EGFR is expressed in 29 of 60 of human gastric cancer. In addition, DNEGFR induces G(0)/G(1) arrest by decreasing expression of phosphorylated retinoblastoma protein, phosphorylated GSK-3 beta, cyclin D1, and by increasing expression of p21 and p27 in human gastric cancer cell lines SGC-7901 and NCI-N87. Finally, DNEGFR induces apoptosis in these cells. Our results indicate that DNFGFR may provide promising treatment strategy for a subgroup of human gastric cancers that express EGFR.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30972872]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学4 区药学4 区核医学
最新[2023]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学4 区药学4 区核医学
JCR分区：
出版当年[2012]版：
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3MEDICINE, RESEARCH & EXPERIMENTALQ3PHARMACOLOGY & PHARMACYQ3ONCOLOGY
最新[2023]版：
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3MEDICINE, RESEARCH & EXPERIMENTALQ3ONCOLOGYQ3PHARMACOLOGY & PHARMACY
第一作者机构：[1]Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
通讯作者：
通讯机构：[1]Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.[*1]Department of Gastrointestinal Surgery, The First Affiliated Hospital, Chongqing Medical University ,No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China
推荐引用方式(GB/T 7714)：
Liao Gang,Wang Ziwei,Zhang Neng,et al.Dominant Negative Epidermal Growth Factor Receptor Inhibits Growth of Human Gastric Cancer Cells by Inducing Cell Cycle Arrest and Apoptosis[J].CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS.2013,28(6):450-458.doi:10.1089/cbr.2012.1399.
APA：
Liao, Gang,Wang, Ziwei,Zhang, Neng&Dong, Pujiang.(2013).Dominant Negative Epidermal Growth Factor Receptor Inhibits Growth of Human Gastric Cancer Cells by Inducing Cell Cycle Arrest and Apoptosis.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,28,(6)
MLA：
Liao, Gang,et al."Dominant Negative Epidermal Growth Factor Receptor Inhibits Growth of Human Gastric Cancer Cells by Inducing Cell Cycle Arrest and Apoptosis".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 28..6(2013):450-458
