机构：[1]Department of Infection Prevention and Control, The First People’s Hospital of Kunming City, Kunming, Yunnan, People’s Republic of China[2]Yunnan Provincial Center of Disease Control and Prevention, Kunming, Yunnan, People’s Republic of China[3]Department of Clinical Laboratory, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China医技片检验科云南省第一人民医院[4]Department of Clinical Laboratory, the First People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China医技片检验科云南省第一人民医院
Purpose: Salmonella enterica serovar Typhimurium infection is common in foodborne diseases, but its isolation from surgical incisions is rare. Our aim in this study was to trace the transmission source of a surgical incision infected with S. Typhimurium in a Yunnan Province hospital patient and elucidate the underlying molecular mechanisms of antibiotic resistance. Methods: Primers were designed to amplify the drug-resistance genes using polymerase chain reaction (PCR). Susceptibility to antibiotics was determined using Etest strips. Macrorestriction profiles were analyzed using pulsed-field gel electrophoresis (PFGE) and XbaI. The two isolates were characterized using agglutination tests and multilocus sequence typing (MLST). Results: MLST analysis revealed that S. Typhimurium isolates SM043 and SM080 belonged to the same genotype, ST34, and PFGE revealed that SM043 and SM080 had high similarity. The isolates were both resistant to third-generation cephalosporins. SM043 harbored the antibiotic resistance genes blaCTX-M-15, blaTEM-1, qnrS-1, qnrB, and acc-3, whereas blaCTX-M-15, blaTEM-1, blaCMY-2, qnrS-1, and acc-3 were detected in SM080. Conclusion: The surgical incision infection by S. Typhimurium may have been hospital-acquired. Thus, it is critical to strengthen hospital sanitation by addressing hand hygiene and sterilization of the operational environment to avoid outbreaks of nosocomial Salmonella infections.
基金：
Yunnan Science and
Technology Commission from Yunnan provincial Science
and Technology Department and Kunming Medical
University, grant number 2018FE001(−115), Health commission
of Yunnan province talent program, grant number
H-2017027, and Yunnan provincial Internal Research
Institutions, grant numbers 2017NS230 and 2018NS0250.
第一作者机构：[1]Department of Infection Prevention and Control, The First People’s Hospital of Kunming City, Kunming, Yunnan, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[3]Department of Clinical Laboratory, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China[4]Department of Clinical Laboratory, the First People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China
推荐引用方式(GB/T 7714)：
Qin Haiyan,Guo Yidan,Li Yikun,et al.Molecular Relatedness of Salmonella enterica Typhimurium Isolates from Feces and an Infected Surgical Wound[J].INFECTION AND DRUG RESISTANCE.2020,13:2139-2144.doi:10.2147/IDR.S251695.
APA：
Qin, Haiyan,Guo, Yidan,Li, Yikun&Zheng, Rui.(2020).Molecular Relatedness of Salmonella enterica Typhimurium Isolates from Feces and an Infected Surgical Wound.INFECTION AND DRUG RESISTANCE,13,
MLA：
Qin, Haiyan,et al."Molecular Relatedness of Salmonella enterica Typhimurium Isolates from Feces and an Infected Surgical Wound".INFECTION AND DRUG RESISTANCE 13.(2020):2139-2144
