机构：[1]Pharmaceutical College and Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, Kunming, Yunnan 650500[2]Key Laboratory of Bio‑active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology[3]The Emergency Department, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032门急诊片急诊内科云南省第一人民医院[4]The Clinical Laboratory Department, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000, P.R. China
Osteoporosis (OP) is a chronic bone disease that affects individuals worldwide. Osteoporosis is primarily asymptomatic, and patients with OP suffer from pain, inconvenience, economic pressure and osteoporotic fracture (OPF). Osteoking, a Traditional Chinese Medicine compound that originates from the Yi ethnic group, has been used for a number of years to treat fractures. In our previous study, osteoking exhibited therapeutic effects on rats with OPF by promoting calcium deposition. Based on bioinformatics and network pharmacology analyses of a component-target-disease database, heat shock protein HSP 90-beta (HSP90-beta), also known as HSP90-beta, was identified to be a key target of osteoking in OP. High HSP90-beta expression levels were observed in osteoporotic rats and rat bone mesenchymal stem cells (rBMSCs) following osteoking treatment. After 12 weeks of administration in vivo, there was increased bone mineral density (BMD) (P<0.05), increased bone alkaline phosphatase (P<0.05), and improved bone microstructure in the osteoking group compared with those of the negative control group. In vitro, increased calcium deposition in rBMSCs was observed after 4 weeks of osteoking treatment. These results suggest that the mechanisms of osteoking are closely associated with HSP90-beta and activate the bone morphogenetic protein (BMP) signalling pathway, primarily through BMP-2. Osteoking treatment improves OP in rats by enhancing HSP90-beta expression.
基金：
National Science Foundation of China-Yunnan Province Joint Fund [U1502227]
第一作者机构：[1]Pharmaceutical College and Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, Kunming, Yunnan 650500[2]Key Laboratory of Bio‑active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology[3]The Emergency Department, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032
共同第一作者：
通讯作者：
通讯机构：[1]Pharmaceutical College and Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, Kunming, Yunnan 650500[3]The Emergency Department, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032[*1]Pharmaceutical College and Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, 1168 Chunrong Street, Kunming, Yunnan 650500, P.R. China[*2]The Emergency Department, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Street, Kunming, Yunnan 650032, P.R. China
推荐引用方式(GB/T 7714)：
Sun Yan,Chen Ran,Zhu Di,et al.Osteoking improves OP rat by enhancing HSP90-beta expression[J].INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE.2020,45(5):1543-1553.doi:10.3892/ijmm.2020.4529.
APA：
Sun, Yan,Chen, Ran,Zhu, Di,Shen, Zhi-Qiang,Zhao, Hong-Bin&Lee, Wen-Hui.(2020).Osteoking improves OP rat by enhancing HSP90-beta expression.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,45,(5)
MLA：
Sun, Yan,et al."Osteoking improves OP rat by enhancing HSP90-beta expression".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 45..5(2020):1543-1553
