机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan 650032, China[3]Clinical Laboratory, First People’s Hospital of Yunnan, Kunming, Yunnan 650032, China
Antimicrobial peptides (AMPs) are a class of templates with application potential for drug development. Amphibians are important sources of AMPs. Duttaphrynus melanostictus is the main source of traditional Chinese medicine "Chansu", which has anti-infection effect while without a clear mechanism. This study aimed to find the cathelicidin peptide in D. melanostictus and then investigate the activity in vivo and in vitro, and an AMP-encoding gene (cathelicidin-DM, GenBank: KJ820824.1) was obtained from the constructed cDNA library of D. melanostictus. The MIC test and SYTOX Green uptake were used for the evaluation of the bactericidal capacity and mechanisms. The serum stability tests were used for the evaluation of the application potential. The skin wound infection model and in vivo imaging were used for in vitro application of possibility evaluation. The results showed that cathelicidin-DM was a 37 amino acid AMP with good bactericidal ability, which was similar to melittin: both can kill bacteria within 15 min. Moreover, cathelicidin-DM exhibits good therapeutic potential in the mouse wound infection model, and it can be enriched to the site of infection for treatment. Thus, cathelicidin-DM could be a new template for antimicrobial drug development given its good antibacterial activity in vivo and in vitro.
基金：
This research was funded by Yunnan Science and Technology
Commission, grant number 2015BC001, and Yunnan Science
and Technology Commission, grant number 2015DH010, and
National Natural Science Foundation of China, grant number
31860607.
第一作者机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan 650032, China
共同第一作者：
通讯作者：
通讯机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan 650032, China[3]Clinical Laboratory, First People’s Hospital of Yunnan, Kunming, Yunnan 650032, China[*1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[*2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan 650032, China[*3]Clinical Laboratory, First People’s Hospital of Yunnan, Kunming, Yunnan 650032, China
推荐引用方式(GB/T 7714)：
Yaoqiang Shi,Chao Li,Mei Wang,et al.Cathelicidin-DM is an Antimicrobial Peptide from Duttaphrynus melanostictus and Has Wound-Healing Therapeutic Potential[J].ACS OMEGA.2020,5(16):9301-9310.doi:10.1021/acsomega.0c00189.
APA：
Yaoqiang Shi,Chao Li,Mei Wang,Zijun Chen,Ying Luo...&A-Mei Zhang.(2020).Cathelicidin-DM is an Antimicrobial Peptide from Duttaphrynus melanostictus and Has Wound-Healing Therapeutic Potential.ACS OMEGA,5,(16)
MLA：
Yaoqiang Shi,et al."Cathelicidin-DM is an Antimicrobial Peptide from Duttaphrynus melanostictus and Has Wound-Healing Therapeutic Potential".ACS OMEGA 5..16(2020):9301-9310
