Objective: Bone-marrow-derived endothelial progenitor cells (EPCs) can accelerate the dissolution of thrombi. However, EPC functions are weakened in deep vein thrombosis (DVT), and miR-130a-3p is downregulated in DVT. As little is known about the function of miR-130a-3p in EPCs, we aimed to explore the effects of miR-130a-3p on EPC functions and the mechanisms of miR-130a-3p regulation of EPCs in DVT. Methods: The EPCs were transfected with miR-130a-3p mimics or miR-130a-3p inhibitor. Migration and angiogenesis of EPCs were detected by wound healing, Transwell, and tube formation assays. Dual luciferase assay was used to test the relation of miR-130a-3p and phosphatase and tensin homolog (PTEN). Protein and mRNA levels of associated genes were measured by western blotting (WB) and qRT-PCR. Results: miR-103a-3p could promote EPC migration and angiogenesis, and it was also downregulated in EPCs isolated from DVT patients. Moreover, PTEN was a target of miR-130a-3p. Upregulation of PTEN rescued the auxoaction of miR-130a-3p in EPC function. Conclusions: Downregulation of miR-103a-3p contributes to EPC dysfunction in DVT via targeting PTEN. Thus, miR-130a-3p may be a potential target for DVT treatment.
基金：
Yunnan Scientific and Technology Committee and Kunming Medical University, China [2018FE001(-299)]; Medical Discipline Leader Training Program of Yunnan Provincial Commission of Health and Family Planning [D201656]
第一作者机构：[1]Kunming Univ Sci & Technol, Peoples Hosp Yunnan Prov 1, Dept Gen Surg, Affiliated Hosp, 157 Tinbi Rd, Kunming 650032, Yunnan, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Kunming Univ Sci & Technol, Peoples Hosp Yunnan Prov 1, Dept Gen Surg, Affiliated Hosp, 157 Tinbi Rd, Kunming 650032, Yunnan, Peoples R China[*1]Department of General Surgery, the First People’s Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Science and Technology, No. 157 Jinbi Road, Kunming, 650032 Yunnan, China
推荐引用方式(GB/T 7714)：
Zhang Peng,Zhao Quan,Gong Kunmei,et al.Downregulation of miR-103a-3p Contributes to Endothelial Progenitor Cell Dysfunction in Deep Vein Thrombosis Through PTEN Targeting[J].ANNALS OF VASCULAR SURGERY.2020,64:339-346.doi:10.1016/j.avsg.2019.10.048.
APA：
Zhang, Peng,Zhao, Quan,Gong, Kunmei,Long, Yaxin,Zhang, Jian...&Guo, Xiaodong.(2020).Downregulation of miR-103a-3p Contributes to Endothelial Progenitor Cell Dysfunction in Deep Vein Thrombosis Through PTEN Targeting.ANNALS OF VASCULAR SURGERY,64,
MLA：
Zhang, Peng,et al."Downregulation of miR-103a-3p Contributes to Endothelial Progenitor Cell Dysfunction in Deep Vein Thrombosis Through PTEN Targeting".ANNALS OF VASCULAR SURGERY 64.(2020):339-346
