机构：[1]Department of Thoracic Surgery, First People's Hospital of Yunnan Province, Kunming 650031, China外科片胸外科云南省第一人民医院[2]Department of Medical Oncology, First People’s Hospital of Yunnan Province, Kunming 650031, China内科片肿瘤内科云南省第一人民医院[3]Anesthesia department, First People’s Hospital of Yunnan Province, Kunming 650031, China外科片麻醉手术科云南省第一人民医院[4]Department of thoracicsurgery, Kailuan general hospital affiliated to north China university of technology, Tangshang 063000, China.[5]Medical School of Kunming University of Science and Technology, Kunming 650031, China
Long noncoding RNA 01296 (Lnc01296) is dys-regulated in malignant tumors. However, the detailed effect of Linc01296 on hepatocellular carcinoma (HCC) remains largely unknown. In this study, we identified the biological role of Linc01296 in HCC. The levels of Linc01296 in HCC tissues and a panel of cell lines were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of Linc01296 on HCC progression were explored using a Cell Counting Kit-8 (CCK-8), flow cytometry, migration and Transwell invasion assays. The interactions among Linc01296, miR-26a and PTEN were determined using luciferase, RNA immunoprecipitation (RIP) and Western blot assays. Tumor xenograft models were utilized to confirm the in vivo functional roles of Linc01296 in HCC development. Linc01296 expression was increased in both HCC tissue samples and cell lines. Knockdown of Linc01296 suppressed HCC cell processes, such as proliferation, migration and invasion, and enhanced apoptosis in vitro; these effects were reversed by a miR-26a mimic or PTEN overexpression. Furthermore, knockdown of Linc01296 suppressed HCC growth in vivo. These findings indicated that Linc01296 is involved in HCC progression via regulating miR-26a/PTEN.
基金：
Huimin Special Science and Technology [2017ZF026]; medical and health units of research institutions for scientific research projects in Yunnan Province [2017NS256]
第一作者机构：[1]Department of Thoracic Surgery, First People's Hospital of Yunnan Province, Kunming 650031, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, First People's Hospital of Yunnan Province, Kunming 650031, China[5]Medical School of Kunming University of Science and Technology, Kunming 650031, China
推荐引用方式(GB/T 7714)：
Zhang Libin,Hu Jing,Hao Menghui,et al.Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis[J].BIOLOGICAL CHEMISTRY.2020,401(3):407-416.doi:10.1515/hsz-2019-0231.
APA：
Zhang, Libin,Hu, Jing,Hao, Menghui&Bu, Liang.(2020).Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis.BIOLOGICAL CHEMISTRY,401,(3)
MLA：
Zhang, Libin,et al."Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis".BIOLOGICAL CHEMISTRY 401..3(2020):407-416
