机构：[1]Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, China[2]Department of Stomatology, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China门急诊片口腔医学中心云南省第一人民医院[3]Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and Academic Center for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Amsterdam Movement Science, Amsterdam, The Netherlands[4]Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou, China南方医科大学珠江医院[5]Department of Oral Implantology and Prosthetic Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands[6]Translational Research Centre of Regenerative Medicine and 3D Printing Technologies of Guangzhou Medical University, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Objectives Mesenchymal stem cells (MSCs) based therapy for bone regeneration has been regarded as a promising method in the clinic. However, hBMSCs with invasive harvesting process and undesirable proliferation rate hinder the extensive usage. HUCMSCs of easier access and excellent performances provide an alternative for the fabrication of tissue-engineered bone construct. Evidence suggested the osteogenesis ability of hUCMSCs was weaker than that of hBMSCs. To address this issue, a co-culture strategy of osteogenically and angiogenically induced hUCMSCs has been proposed since thorough vascularization facilitates the blood-borne nutrition and oxygen to transport in the scaffold, synergistically expediting the process of ossification. Materials and methods Herein, we used osteogenic- and angiogenic-differentiated hUCMSCs for co-culture in screened culture medium to elevate the osteogenic capacity with in vitro studies and finally coupled with 3D TCP scaffold to repair rat's critical-sized calvarial bone defect. By dual-directional induction, hUCMSCs could differentiate into osteoblasts and endothelial cells, respectively. To optimize the co-culture condition, gradient ratios of dual-directional differentiated hUCMSCs co-cultured under different medium were studied to determine the appropriate condition. Results It revealed that the osteogenic- and angiogenic-induced hUCMSCs mixed with the ratio of 3:1 co-cultured in the mixed medium of osteogenic induction medium to endothelial cell induction medium of 3:1 possessed more mineralization nodules. Similarly, ALP and osteogenesis/angiogenesis-related genes expressions were relatively higher. Further evidence of bone defect repair with 3D printed TCP of 3:1 group exhibited better restoration outcomes. Conclusions Our work demonstrated a favourable and convenient approach of dual-directional differentiated hUCMSCs co-culture to improve the osteogenesis, establishing a novel way to fabricate tissue-engineered bone graft with 3D TCP for large bone defect augmentation.
基金：
Joint Fund for Applied Basic Research of
Yunnan Provincial Science and Technology
Department-Kunming Medical School,
Grant/Award Number: 2017FE468-168;
International Team for Implantology,
Grant/Award Number: 881_2012;
National Major Science and Technology
Project of China, Grant/Award Number:
2016YFC1102900; National Nature
Science Foundation of China, Grant/Award
Number: 81671029; Guangzhou Science,
Technology and Innovation Commission,
Grant/Award Number: 201704030024
and 201803040008; Bureau of Education
of Guangzhou Municipality, Grant/Award
Number: 1201610458
第一作者机构：[1]Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, China[2]Department of Stomatology, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, China[6]Translational Research Centre of Regenerative Medicine and 3D Printing Technologies of Guangzhou Medical University, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China[*1]Affiliated Stomatological Hospital of Guangzhou Medical University, No. 31, Huangsha Avenue, Liwan District, Guangzhou 510140, China.[*2]The Third Affiliated Hospital of Guangzhou Medical University, No. 63, Duobao Road, Liwan District, Guangzhou 510150, China.
推荐引用方式(GB/T 7714)：
Rong Qiong,Li Shuyi,Zhou Yang,et al.A novel method to improve the osteogenesis capacity of hUCMSCs with dual-directional pre-induction under screened co-culture conditions[J].CELL PROLIFERATION.2020,53(2):doi:10.1111/cpr.12740.
APA：
Rong, Qiong,Li, Shuyi,Zhou, Yang,Geng, Yuanming,Liu, Shangbin...&Zhou, Miao.(2020).A novel method to improve the osteogenesis capacity of hUCMSCs with dual-directional pre-induction under screened co-culture conditions.CELL PROLIFERATION,53,(2)
MLA：
Rong, Qiong,et al."A novel method to improve the osteogenesis capacity of hUCMSCs with dual-directional pre-induction under screened co-culture conditions".CELL PROLIFERATION 53..2(2020)
生物学