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PLCE1 inhibits apoptosis of non-small cell lung cancer via promoting PTEN methylation

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收录情况： ◇ SCIE

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机构： [1]Department of Respiratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, China

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关键词： NSCLC PLCE1 PTEN Cell apoptosis

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OBJECTIVE: To explore the role of phospholipase C epsilon 1 (PLCE1) in regulating cell apoptosis of non-small cell lung cancer (NSCLC) and its underlying mechanism. PATIENTS AND METHODS: The mRNA and protein levels of PLCE1 in NSCLC tissues, adjacent normal tissues and NSCLC cell lines (A549 and H1299) were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. Methylation status in the promoter region of PTEN in NSCLC cells was accessed using the relative commercial kit. Cell apoptosis after transfection of PLCE1 siRNA in NSCLC cells was detected by flow cytometry. Protein expressions of apoptosis-related genes in NSCLC cells after altering PLCE1 expression were detected by Western blot. RESULTS: PLCE1 was highly expressed in NSCLC tissues and cell lines than that of controls. PLCE1 knockdown promoted PTEN expression and inhibited methylation in H1299 cells. Transfection of PLCE1 siRNA in NSCLC cells remarkably induced cell apoptosis. CONCLUSIONS: PLCE1 inhibits cell apoptosis of NSCLC by promoting PTEN methylation.

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出版当年[2019]版：

大类 | 4 区医学

小类 | 4 区药学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区药学

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出版当年[2018]版：

Q2 PHARMACOLOGY & PHARMACY

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无

影响因子： 0 最新[2023版] 0 最新五年平均 2.721 出版当年[2018版] 2.141 出版当年五年平均 2.387 出版前一年[2017版] 3.024 出版后一年[2019版]

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第一作者机构： [1]Department of Respiratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, China

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