机构：[1]Department of Thoracic Surgery, First People’s Hospital of Yunnan Province, Kunming, China外科片胸外科云南省第一人民医院[2]Department of Medical Oncology, First People’s Hospital of Yunnan Province, Kunming, China内科片肿瘤内科云南省第一人民医院[3]Anesthesia Department, First People’s Hospital of Yunnan Province, Kunming, China外科片麻醉手术科云南省第一人民医院[4]Department of Thoracic Surgery, Kailuan General Hospital affiliated to North China University of Technology, Tangshang, China[5]Medical School, Kunming University of Science and Technology, Kunming, China
Long noncoding RNA, long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) was showed to be involved in cancer development. However, the biological effect of LINC-PINT on non-small cell lung cancer (NSCLC) remains unknown. Here, we aimed to investigate the role and underlying mechanism of LINC-PINT in NSCLC. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the level of LINC-PINT in NSCLC tissues and cell lines. Cell counting kit-8 (CCK-8), flow cytometry, migration and transwell invasion assays were used to investigate cell proliferation, cell cycle, cell migration and invasion, respectively. The targets of LINC-PINT were verified by both luciferase reporter assay and RNA immunoprecipitation assay. Tumour xenografts were used to reveal the effect of LINC-PINT on tumourigenesis in vivo. We observed that LINC-PINT expression increased in both NSCLC tissues and cell lines. Function assays exhibited that LINC-PINT reduced NSCLC cell proliferation, cell cycle, cell migration and invasion in vitro. We also indicated that LINC-PINT mediated inhibitory effect on cell proliferation, cell cycle, cell migration and invasion by miR-208a-3p/programmed cell death 4 (PDCD4) in NSCLC cells. These findings indicated that LINC-PINT functions as a tumour-suppressor that exerts important regulatory roles in NSCLC progression by sponging miR-208a-3p/PDCD4.
基金：
This project was supported by a grant from the Huimin Special Science
and Technology [2017ZF026], Medical and Health Unit with Research
Institutions of Scientific Research Projects in Yunnan
Province [2017NS256].
第一作者机构：[1]Department of Thoracic Surgery, First People’s Hospital of Yunnan Province, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, First People’s Hospital of Yunnan Province, Kunming, China[4]Department of Thoracic Surgery, Kailuan General Hospital affiliated to North China University of Technology, Tangshang, China[5]Medical School, Kunming University of Science and Technology, Kunming, China[*1]Department of Thoracic Surgery, Kailuan General Hospital affiliated to North China University of Technology, Tangshang 063000, China[*2]Department of thoracic surgery, First People’s Hospital of Yunnan Province, Kunming 650031, China
推荐引用方式(GB/T 7714)：
Zhang Libin,Hu Jing,Li Jiagui,et al.Long noncoding RNA LINC-PINT inhibits non-small cell lung cancer progression through sponging miR-218-5p/PDCD4[J].ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY.2019,47(1):1595-1602.doi:10.1080/21691401.2019.1605371.
APA：
Zhang, Libin,Hu, Jing,Li, Jiagui,Yang, Qiuju,Hao, Menghui&Bu, Liang.(2019).Long noncoding RNA LINC-PINT inhibits non-small cell lung cancer progression through sponging miR-218-5p/PDCD4.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,47,(1)
MLA：
Zhang, Libin,et al."Long noncoding RNA LINC-PINT inhibits non-small cell lung cancer progression through sponging miR-218-5p/PDCD4".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 47..1(2019):1595-1602
