机构：[1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P.R. China.[2]Department of Respiration, First People’s Hospital of Yunnan Province, Kunming, Yunnan, P.R. China.内科片呼吸与危重症医学科云南省第一人民医院[3]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, P.R. China.[4]School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan, P.R. China.[5]Technology Transfer Center, Kunming Medical University, Kunming, Yunnan, P.R. China.[6]The HKU–Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Outbreaks of avian influenza virus continue to pose threats to human health. Animal models such as the mouse, ferret, and macaque are used to understand the pathogenesis of avian influenza virus infection in humans. We previously reported that the tree shrew (Tupaia belangeri, family Tupaiidae), which is regarded as a "low-level primate", has a2,3- and a2,6-linked sialic acid receptor distributions similar to those of humans and is potentially a useful mammalian model for studying mild human influenza (H1N1) virus infection. In this study, we used the tree shrew experimental model to investigate the pathogenesis of avian influenza A (H9N2) virus infection and the effect of the E627K mutation in the PB2 gene, an adaptation to mammalian hosts. Evidence of disease, virus titers in the upper and lower respiratory tract, histopathology and induction of proinflammatory cytokines are described. We also established ex vivo culture models of tree shrew respiratory tissues to study the tropism and replication of the H9N2 virus. Our results demonstrated that the tree shrew is a viable new in vivo experimental model for avian influenza research that provides results comparable to those observed in ferrets. The disease spectrum and pathogenesis in tree shrews correlate well with what is observed in humans.
基金：
National Natural Science Foundation of China
(81471937); by the Yangcheng Scholars Research Fund for Guangzhou
Municipal Universities (1201561571); by the Science and Technology Program
of Guangzhou Municipal Universities (1201410111); by the Science and
Technology Program of Guangzhou (201504010032); and by the Research
Grants Council of the Hong Kong Special Administrative Region, China,
through the Theme-Based Research Scheme (Ref: T11-705/14N).
第一作者机构：[1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P.R. China.
通讯作者：
通讯机构：[1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P.R. China.[6]The HKU–Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
推荐引用方式(GB/T 7714)：
Runfeng Li,Bing Yuan,Xueshan Xia,et al.Tree shrew as a new animal model to study the pathogenesis of avian influenza (H9N2) virus infection[J].EMERGING MICROBES & INFECTIONS.2018,7:doi:10.1038/s41426-018-0167-1.
APA：
Runfeng Li,Bing Yuan,Xueshan Xia,Sheng Zhang,Qiuling Du...&Zifeng Yang.(2018).Tree shrew as a new animal model to study the pathogenesis of avian influenza (H9N2) virus infection.EMERGING MICROBES & INFECTIONS,7,
MLA：
Runfeng Li,et al."Tree shrew as a new animal model to study the pathogenesis of avian influenza (H9N2) virus infection".EMERGING MICROBES & INFECTIONS 7.(2018)
医学