机构：[1]Department of Respiratory Medicine, The First People’s Hospital of Yunnanprovince, Kunming 650022, China[2]The Affiliated Hospital of KunmingUniversity of Science and Technology, Kunming, Yunnan, China[3]Center ofTree Shrew Germplasm Resources, Institute of Medical Biology, The ChineseAcademy of Medical Science and Peking Union Medical College, Yunnan KeyLaboratory of Vaccine Research and Development on Severe InfectiousDiseases, Yunnan Innovation Team of Standardization and ApplicationResearch in Tree Shrew, Kunming 650118, China[4]Department of AcuteInfectious Diseases Control and Prevention, Yunnan Provincial Centre forDisease Control and Prevention, Kunming 650022, China[5]Centre for SanitaryInspection, Yunnan Provincial Centre for Disease Control and Prevention,Kunming 650022, China[6]The Third Affiliated Hospital of Sun Yat-SenUniversity, Guangzhou 510630, China中山大学附属第三医院[7]Wenshan Centre for Disease Controland Prevention, Wenshan 663000, China[8]Institute of Medical Biology, TheChinese Academy of Medical Science and Peking Union Medical College &Department of Acute Infectious Diseases Control and Prevention, YunnanProvincial Centre for Disease Control and Prevention, Dongsi Street 158,Kunming 650022, China[9]Center of Tree Shrew Germplasm Resources,Institute of Medical Biology, Chinese Academy of Medical Sciences & PekingUnion Medical School, Kunming 650118, China
Background: Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases in the world. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and spa typing methods were used to characterize Staphylococcus aureus isolates from food surveillance during 2013-2015 in southwest China, and Staphylococcal cassette chromosome mec (SCCmec) typing was used for methicillin-resistant S. aureus (MRSA). Isolates were also examined for their antibiotic resistance and carriage of virulence genes. Results: Isolation rate of S. aureus was 2.60% during the three years' surveillance and 29.50% of them were MRSA. All the S. aureus had hla genes (100%), 14.34% of the strains had tst, and 16.73% had PVL. 163 PFGE-Smal patterns, 41 ST types and 36 spa types were obtained for all the S. aureus. Among them, ST6-t701 (13.15%), ST7-t091 (12.75%) , ST59-t437 (9.96%) and ST5-t002 (7.57%) were the prevalent genotypes. Most of MRSA in this study belonged to SCCmec IV and V, accounted for 74.32% and 20.27% respectively. ST6-SCCmec IV-t701 (36.50%) was the most prevalent clone among isolates from food, followed by ST59-SCCmec V-t437 (20.30%), ST5-SCCmec IV-t002 (12.20%) and ST59-SCCmec IV-t437 (12.20%). Some strains had the identical PFGE patterns, ST and spa types with isolates from patients. Conclusions: S. aureus isolated from food in southwest China displayed heterogeneity. Isolates had the same genotype profiles with isolates from patients, indicating high homology.
基金：
Yunnan Science and Technology Talent and
Platform Program (2017HC019) and National Sci-Tech key project
(2017ZX10103010).
第一作者机构：[1]Department of Respiratory Medicine, The First People’s Hospital of Yunnanprovince, Kunming 650022, China[2]The Affiliated Hospital of KunmingUniversity of Science and Technology, Kunming, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[3]Center ofTree Shrew Germplasm Resources, Institute of Medical Biology, The ChineseAcademy of Medical Science and Peking Union Medical College, Yunnan KeyLaboratory of Vaccine Research and Development on Severe InfectiousDiseases, Yunnan Innovation Team of Standardization and ApplicationResearch in Tree Shrew, Kunming 650118, China[4]Department of AcuteInfectious Diseases Control and Prevention, Yunnan Provincial Centre forDisease Control and Prevention, Kunming 650022, China[8]Institute of Medical Biology, TheChinese Academy of Medical Science and Peking Union Medical College &Department of Acute Infectious Diseases Control and Prevention, YunnanProvincial Centre for Disease Control and Prevention, Dongsi Street 158,Kunming 650022, China[9]Center of Tree Shrew Germplasm Resources,Institute of Medical Biology, Chinese Academy of Medical Sciences & PekingUnion Medical School, Kunming 650118, China
推荐引用方式(GB/T 7714)：
Feng Liao,Wenpeng Gu,Zushun Yang,et al.Molecular characteristics of Staphylococcus aureus isolates from food surveillance in southwest China[J].BMC MICROBIOLOGY.2018,18:doi:10.1186/s12866-018-1239-z.
APA：
Feng Liao,Wenpeng Gu,Zushun Yang,Zhishuo Mo,Lu Fan...&Jiejie Dai.(2018).Molecular characteristics of Staphylococcus aureus isolates from food surveillance in southwest China.BMC MICROBIOLOGY,18,
MLA：
Feng Liao,et al."Molecular characteristics of Staphylococcus aureus isolates from food surveillance in southwest China".BMC MICROBIOLOGY 18.(2018)
