Effects of microRNA-292-5p on myocardial ischemia-reperfusion injury through the peroxisome proliferator-activated receptor-alpha/-gamma signaling pathway
机构：[1]Department of Cardiovascular Diseases, The Third People’sHospital of Yunnan Province, The Second Affiliated Hospital ofDali University, 650011 Kunming, People’s Republic of China[2]Pathogenic Organisms Department of Experimental Center,School of Basic Medical Sciences, Kunming Medical University,650500 Kunming, People’s Republic of China[3]Clinical Laboratory, The First People’s Hospital of YunnanProvince, 650032 Kunming, People’s Republic of China[4]Department of Geriatrics, The First People’s Hospital of YunnanProvince, 650032 Kunming, People’s Republic of China[5]Clinical Medicine College, Dali University, 671003 Dali, People’sRepublic of China[6]School of Pharmaceutical Sciences & Yunnan Key Laboratory ofPharmacology for Natural Products, Kunming Medical University,650500 Kunming, People’s Republic of China
Ischemia-reperfusion injury (IRI) is a major cause of cardiac damage following various pathological processes, such as free radical damage and cell apoptosis. This study aims to investigate whether microRNA-292-5p (miR-292-5p) protects against myocardial ischemia-reperfusion injury (IRI) via the peroxisome proliferator-activated receptor (PPAR)-alpha/-gamma signaling pathway in myocardial IRI mice models. Mouse models of myocardial IRI were established. Adult male C57BL/6 mice were divided into different groups. The hemodynamic indexes, levels of related inflammatory factors and serum myocardial enzymes, and malondialdehyde (MDA) content and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected. The 2,3,5-triphenyltetrazolium chloride (TTC) staining was applied to determine infarct size. TUNEL staining was used to detect cardiomyocyte apoptosis. RT-qPCR and western blotting were performed to measure the related gene expressions. Compared with the model group and the T0070907 + miR-292-5p inhibitor, the miR-292-5p inhibitor group exhibited decreased incidence and duration time of ventricular tachycardia and ventricular fibrillation, serum myocardial enzymes, TNF-alpha, IL-6, IL-1 beta, MDA, cardiomyocyte apoptosis, expressions of Bax and p53 in addition to increased SOD and GSH-Px activity, and increased expressions of Bcl-2, PPAR alpha, PPAR gamma, PLIN5, AQP7, and PCK1. The T0070907 group exhibited opposite results compared to the miR-292-5p inhibitor group. The results indicate that miR-292-5p downregulation protects against myocardial IRI through activation of the PPAR-alpha/PPAR-gamma signaling pathway.
基金：
Yunnan Provincial Science and Technology Plan Project on Applied Basic Research [2012FB088, 2017FF117 (-064)]; Yunnan Provincial Applied Basic Research Program-Basic Research Projects [2014NS243]
第一作者机构：[1]Department of Cardiovascular Diseases, The Third People’sHospital of Yunnan Province, The Second Affiliated Hospital ofDali University, 650011 Kunming, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[6]School of Pharmaceutical Sciences & Yunnan Key Laboratory ofPharmacology for Natural Products, Kunming Medical University,650500 Kunming, People’s Republic of China
推荐引用方式(GB/T 7714)：
Zhu Zhen-Dong,Ye Ji-Yun,Niu Hua,et al.Effects of microRNA-292-5p on myocardial ischemia-reperfusion injury through the peroxisome proliferator-activated receptor-alpha/-gamma signaling pathway[J].GENE THERAPY.2018,25(3):234-248.doi:10.1038/s41434-018-0014-y.
APA：
Zhu, Zhen-Dong,Ye, Ji-Yun,Niu, Hua,Ma, Yu-Mei,Fu, Xue-Mei...&Zhang, Xuan.(2018).Effects of microRNA-292-5p on myocardial ischemia-reperfusion injury through the peroxisome proliferator-activated receptor-alpha/-gamma signaling pathway.GENE THERAPY,25,(3)
MLA：
Zhu, Zhen-Dong,et al."Effects of microRNA-292-5p on myocardial ischemia-reperfusion injury through the peroxisome proliferator-activated receptor-alpha/-gamma signaling pathway".GENE THERAPY 25..3(2018):234-248
