Osteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR. Using a dual-luciferase reporter assay, we found that miR-142-5p can bind to and decrease expression of VCAM-1 mRNA. Such reduction was prohibited when the miR-142-5p binding site in VCAM-1 3'UTR was deleted, and Western blotting analyses validated the fact that miR-142-5p inhibited the expression ofVCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. Collectively, these data indicated the important role of miR-142-5p in osteoporosis development involving targeting VCAM-1 and inhibiting BMMSC migration.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81660156, 81760136, 81671928]; Joint Special Fund of Applied Fundamental Research of Kunming Medical University - Science and Technology Office of Yunnan [2014FZ048, 2017FE468-181]; Australian National Health Medical Research Council (NHMRC) Senior Research FellowshipsNational Health and Medical Research Council of Australia [1042105]
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外文
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中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2023]版：
无
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出版当年[2017]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Kunming Med Univ, Affiliated Hosp 6, Peoples Hosp Yuxi City, Dept Orthoped Surg, Yuxi 653100, Yunnan, Peoples R China
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推荐引用方式(GB/T 7714)：
Teng Zhaowei,Xie Xueguan,Zhu Yun,et al.miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration[J].BIOMED RESEARCH INTERNATIONAL.2018,2018:doi:10.1155/2018/3274641.
APA：
Teng, Zhaowei,Xie, Xueguan,Zhu, Yun,Liu, Jianping,Hu, Xingbo...&Xian, Cory J..(2018).miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration.BIOMED RESEARCH INTERNATIONAL,2018,
MLA：
Teng, Zhaowei,et al."miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration".BIOMED RESEARCH INTERNATIONAL 2018.(2018)
