机构：[1]Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China[2]School of Medicine, Yunnan University, Kunming 650091, China[3]Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China[4]Department of Hepatic and Bile Duct Surgery, The First People’s Hospital of Yunnan Province, Kunming 650032, China外科片肝胆外科云南省第一人民医院[5]Yunnan Provincial Academy of Science and Technology, Kunming 650500, China[6]Kunming Ennovate Institute of Bioscience, Kunming 650500, China[7]Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Mesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are two types of cryoprotectants widely used for cell vitrification. However, the effects of DMSO and EG on the biological characteristics and transcriptome profiles of MSCs after cryopreservation remain unknown. In the present study, the viability, immunophenotype of cell surface markers, proliferation, differentiation potency, and global gene expression of rhesus macaque bone marrow-derived MSCs vitrified using DMSO and EG were studied. The results showed that vitrification did not affect the morphology, surface markers, and differentiation of the MSCs, and compared to DMSO, EG better protected cell viability and proliferation. Most importantly, vitrification resulted in changes in a large number of transcripts of MSCs either preserved using DMSO or EG. This report is the first to examine the effects of DMSO and EG on global gene expression in stem cells. These results will be beneficial to understanding the biological process involved in MSC vitrification and will contribute to improving cryopreservation protocols that maintain transcriptomic identity with high cryosurvival for preclinical research and clinical long-term storage.
基金：
National Key Research and Development Program of China [2016YFA0101403]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31660346, 81560043]; Yunnan young leaders in academic and technical talents project [2012HB040]; Yunnan Provincial Health Science and Technology program [2017NS248]
第一作者机构：[1]Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China[2]School of Medicine, Yunnan University, Kunming 650091, China[3]Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China
共同第一作者：
通讯作者：
通讯机构：[1]Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China[5]Yunnan Provincial Academy of Science and Technology, Kunming 650500, China[6]Kunming Ennovate Institute of Bioscience, Kunming 650500, China
推荐引用方式(GB/T 7714)：
Fu Xufeng,Yan Yaping,Li Shanshan,et al.Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression[J].STEM CELLS INTERNATIONAL.2017,2017:doi:10.1155/2017/3893691.
APA：
Fu, Xufeng,Yan, Yaping,Li, Shanshan,Wang, Junfeng,Jiang, Bin...&Si, Wei.(2017).Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression.STEM CELLS INTERNATIONAL,2017,
MLA：
Fu, Xufeng,et al."Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression".STEM CELLS INTERNATIONAL 2017.(2017)
