机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan, China,[2]Cadres Treatment Section & ThoracicSurgery Department, the First People’s Hospital of Yunnan Province, Kunming, Yunnan, China,云南省第一人民医院[3]School of Basic Medical Sciences, Yunnan University of Traditional ChineseMedicine, Kunming, Yunnan, China,[4]College of Humanities & Social Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China,[5]Engineering Research Center ofBiopolymer Functional Materials of Yunnan, Yunnan University of Nationalities, Kunming, Yunnan, China,[6]Key Laboratory of Animal Models and Human DiseaseMechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China
Spiders have evolved highly selective toxins for insects. There are many insecticidal neurotoxins in spider venoms. Although a large amount of work has been done to focus on neurotoxicity of spider components, little information, which is related with effects of spider toxins on tumor cell proliferation and cytotoxicity, is available for Brachypelma albopilosum venom. In this work, a novel spider neurotoxin (brachyin) was identified and characterized from venoms of the spider, Brachypelma albopilosum. Brachyin is composed of 41 amino acid residues with the sequence of CLGENVPCDKDRPNCCSRYECLEPT-GYGWWYASYYCYKKRS. There are six cysteines in this sequence, which form three disulfided bridges. The serine residue at the C-terminus is amidated. Brachyin showed strong lethal effects on American cockroaches (Periplaneta americana) and Tenebrio molitor (common mealbeetle). This neurotoxin also showed significant analgesic effects in mice models including abdominal writhing induced by acetic acid and formalin-induced paw licking tests. It was interesting that brachyin exerted marked inhibition on tumor cell proliferation.
基金：
ational Natural Science Foundation of China (31160198, 31360417), the Applied Basic Research Key Project of Yunnan
(2013FA039), the Applied Basic Research Project of Yunnan (2011FB084), the High-End Technology Professionals Introduction Plan in Yunnan province
(2010CI119).
第一作者机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan, China,[2]Cadres Treatment Section & ThoracicSurgery Department, the First People’s Hospital of Yunnan Province, Kunming, Yunnan, China,
共同第一作者：
通讯作者：
通讯机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan, China,[2]Cadres Treatment Section & ThoracicSurgery Department, the First People’s Hospital of Yunnan Province, Kunming, Yunnan, China,
推荐引用方式(GB/T 7714)：
Yunhua Zhong,Bo Song,Guoxiang Mo,et al.A Novel Neurotoxin from Venom of the Spider, Brachypelma albopilosum[J].PLOS ONE.2014,9(10):doi:10.1371/journal.pone.0110221.
APA：
Yunhua Zhong,Bo Song,Guoxiang Mo,Mingwei Yuan,Hongli Li...&Qiumin Lu.(2014).A Novel Neurotoxin from Venom of the Spider, Brachypelma albopilosum.PLOS ONE,9,(10)
MLA：
Yunhua Zhong,et al."A Novel Neurotoxin from Venom of the Spider, Brachypelma albopilosum".PLOS ONE 9..10(2014)
