机构：[1]Division of Molecular of Therapy, Center for Advanced Medical Research, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.[2]Department of Cell Processing and Transfusion, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.[3]Department of Hematology, First People Hospital of Yunnan Province, Kunming, China.内科片血液内科云南省第一人民医院[4]Department of Obstetrics, NTT Medical Center Tokyo Hospital, Tokyo, Japan.[5]Tissue Engineering Research Group, Division of Molecular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Background: Umbilical cord Wharton's jelly (WJ) is a rich source of mesenchymal stem cells (MSCs) similar to bone marrow (BM) and adipose tissues. Stage-specific embryonic antigen (SSEA)4 has been reported as a stem cell marker in BM-derived MSCs, but whether SSEA4(+) cells have growth and differentiation advantages over SSEA4(-) cells remains controversial. To gain insight into the role of SSEA4, we studied SSEA4(+) cells in WJ-derived MSCs (WJ-MSCs). Methods: WJ-MSCs were collected by the explant (WJe-MSCs) or collagenase methods (WJc-MSCs) and analyzed by flow cytometry and reverse-transcription polymerase chain reaction (RT-PCR). To evaluate whether culture conditions influenced the SSEA4 expression, WJe-MSCs were cultured in the medium supplemented with different fetal bovine serum (FBS) concentrations. Results: SSEA4 was expressed for a long-term culture. In contrast, SSEA3(+) disappeared rapidly in early passages of the culture. The incidence of SSEA4(+) and SSEA3(+) cells was similar between WJe-MSCs and WJc-MSCs at passages P0-P9, except for transient depletion of SSEA4 expression in early passages of WJe-MSCs. These were CD73(+)CD105(+) cells that express embryonic stem cell markers detected by RT-PCR. No differences in growth and differentiation ability of osteocytes and adipocytes were observed between the sorted SSEA4(+) cells and SSEA4(-) cells. Further, SSEA4 expression in WJe-MSCs was significantly correlated with FBS concentration in the culture medium. Discussion: SSEA4, which may display altered expression profiles in response to culture conditions, may not be an essential marker of WJ-MSC multipotency.
基金：
Ministry of Health, Labor and Welfare, JapanMinistry of Health, Labour and Welfare, Japan; Ministry of Education, Culture, Sports, Science and Technology, JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
第一作者机构：[1]Division of Molecular of Therapy, Center for Advanced Medical Research, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.[2]Department of Cell Processing and Transfusion, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.[3]Department of Hematology, First People Hospital of Yunnan Province, Kunming, China.
通讯作者：
通讯机构：[2]Department of Cell Processing and Transfusion, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.[*1]Department of Cell Processing and Transfusion The Institute of Medical Science The University of Tokyo 4-6-1Shirokanedai, Minato-ku Tokyo 108-8639 Japan
推荐引用方式(GB/T 7714)：
He Haiping,Nagamura-Inoue Tokiko,Tsunoda Hajime,et al.Stage-Specific Embryonic Antigen 4 in Wharton's Jelly-Derived Mesenchymal Stem Cells Is Not a Marker for Proliferation and Multipotency[J].TISSUE ENGINEERING PART A.2014,20(7-8):1314-1324.doi:10.1089/ten.tea.2013.0333.
APA：
He, Haiping,Nagamura-Inoue, Tokiko,Tsunoda, Hajime,Yuzawa, Miki,Yamamoto, Yuki...&Tojo, Arinobu.(2014).Stage-Specific Embryonic Antigen 4 in Wharton's Jelly-Derived Mesenchymal Stem Cells Is Not a Marker for Proliferation and Multipotency.TISSUE ENGINEERING PART A,20,(7-8)
MLA：
He, Haiping,et al."Stage-Specific Embryonic Antigen 4 in Wharton's Jelly-Derived Mesenchymal Stem Cells Is Not a Marker for Proliferation and Multipotency".TISSUE ENGINEERING PART A 20..7-8(2014):1314-1324
