机构：[1]Department of Rehabilitation of Huashan Hospital, Fudan University,Shanghai, China[2]Department of biomedical engineering, Shanghai JiaoTongUniversity, Shanghai, China[3]Department of Biomedical Engineering, StonyBrook University, New York, USA[4]Genetic Diagnosis Center, YunnanProvincial Key Laboratory For Birth Defects and Genetic Diseases, The FirstPeople's Hospital of Yunnan Province, Kunming, China[5]The Yonghe Branchof Huashan Hospital, Fudan University, Shanghai 200032, China
Background: Early exercise after stroke promoted angiogenesis and increased microvessles density. However, whether these newly formatted vessels indeed give rise to functional vascular and improve the cerebral blood flow (CBF) in impaired brain region is still unclear. The present study aimed to determine the effect of early exercise on angiogenesis and CBF in ischemic region. Methods: Adult male Sprague Dawley rats were subjected to 90 min middle cerebral artery occlusion(MCAO) and randomly divided into early exercise and non-exercised control group 24 h later. Two weeks later, CBF in ischemic region was determined by laser speckle flowmetry(LSF). Meantime, micro vessels density, the expression of tie-2, total Akt and phosphorylated Akt (p-Akt), and infarct volume were detected with immunohistochemistry, 2,3,5 triphenyltetrazolium chloride (TTC) staining and western blotting respectively. The function was evaluated by seven point's method. Results: Our results showed that CBF, vessel density and expression of Tie-2, p-Akt in ischemic region were higher in early exercise group compared with those in non-exercise group. Consistent with these results, rats in early exercise group had a significantly reduced infarct volume and better functional outcomes than those in non-exercise group. Conclusions: Our results indicated that early exercise after MCAO improved the CBF in ischemic region, reduced infarct volume and promoted the functional outcomes, the underlying mechanism was correlated with angiogenesis in the ischemic cortex.
基金：
Chinese National Natural Science
Foundation (81272169, 81201502, 81171856, and 81171855).
第一作者机构：[1]Department of Rehabilitation of Huashan Hospital, Fudan University,Shanghai, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Pengyue,Yu Huixian,Zhou Naiyun,et al.Early exercise improves cerebral blood flow through increased angiogenesis in experimental stroke rat model[J].JOURNAL OF NEUROENGINEERING AND REHABILITATION.2013,10:doi:10.1186/1743-0003-10-43.
APA：
Zhang, Pengyue,Yu, Huixian,Zhou, Naiyun,Zhang, Jie,Wu, Yi...&Hu, Yongshan.(2013).Early exercise improves cerebral blood flow through increased angiogenesis in experimental stroke rat model.JOURNAL OF NEUROENGINEERING AND REHABILITATION,10,
MLA：
Zhang, Pengyue,et al."Early exercise improves cerebral blood flow through increased angiogenesis in experimental stroke rat model".JOURNAL OF NEUROENGINEERING AND REHABILITATION 10.(2013)
