机构：[1]Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China,浙江大学医学院附属妇产科医院[2]Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou, China,[3]Department of Obstetrics and Gynaecology, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia V6H 3V5, Canada[4]Department of Reproductive Endocrinology, The First People's Hospital of Yunnan Province, Yunnan, China内科片内分泌科云南省第一人民医院
Background: Progesterone plays an important role in the proliferation and differentiation of human endometrial cells (hECs). Large-dose treatment with progesterone has been used for treatment of endometrial proliferative disorders. However, the mechanisms behind remain unknown. Methods: To investigate the role of cyclin B1 in proliferation and differentiation of hECs in menstrual cycle, the expression of cyclin B1 throughout the menstrual cycle was evaluated in hECs. To determine the effects of progesterone on the proliferation, cell cycle progression and apoptosis of hECs and to test if cyclin B1 is involved in these effects, progesterone and/or Alsterpaullone (Alp, a specific inhibitor of Cyclin B1/Cdc2) were added to primary hECs. Cellular proliferation was evaluated with MTT test, cell cycle with propidium iodide (PI) staining and flow cytometry, apoptosis with FITC-Annexin V and the expression of cyclin B1 with Western blotting. Results: The expression level of cyclin B1 in secretory endometria was significantly lower than in proliferative endometria (p < 0.01). Progesterone significantly inhibited the growth of hECs in a concentration-dependent manner (P < 0.01). The treatment with progesterone significantly decreased the expression of cyclin B1, increased the proportions of cell in G2/M phase, and apoptotic cells (P < 0.05 for all). The presence of Alp significantly enhanced the effects of progesterone on cyclin B1 down-regulation, G2/M cell cycle arrest and induction of apoptosis (P < 0.01 for all). Conclusion: Our findings suggest that cyclin B1 is a critical factor in proliferation and differentiation of hECs. Progesterone may inhibit cell proliferation, mediate G2/M cell cycle arrest and induce apoptosis in hECs via down-regulating Cyclin B1.
基金：
Cai-yun Zhou for technical assistance.
This work was supported by National Basic Research Program of China
(No.973: 2006CB944006 and 2006CB504004).
第一作者机构：[1]Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China,[4]Department of Reproductive Endocrinology, The First People's Hospital of Yunnan Province, Yunnan, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Tang Li,Zhang Yu,Pan Hong,et al.Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell[J].REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY.2009,7:doi:10.1186/1477-7827-7-144.
APA：
Tang, Li,Zhang, Yu,Pan, Hong,Luo, Qiong,Zhu, Xiao-Ming...&Huang, He-Feng.(2009).Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell.REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,7,
MLA：
Tang, Li,et al."Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell".REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 7.(2009)
