机构：[1]Department of Pediatric Neurology,the First People’s Hospital of Yunnan Province, Kunming, Affiliated Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China内科片儿科神经内科云南省第一人民医院[2]Department of Neurology ,Affiliated Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China[3]Department of Pediatrics, the First People’s Hospital of Yunnan Province, Kunming, China内科片儿科云南省第一人民医院
Bacterial meningitis is still one of the most common causes of acquired profound sensorineural deafness in children despite antibiotic treatment. We investigated the neuroprotective effects of brain-derived neurotrophic factor on hearing function in experimental bacterial meningitis. We implanted stainless steel tubes into both cerebral ventricles of Sprague-Dawley rats aged 21 days. Bacterial meningitis was induced by inoculating a strain of serotype III Streptococcus pneumoniae into the cisterna magna. Six micrograms per day of brain-derived neurotrophic factor (groups 1 and 3) or albumin (groups 2 and 4) was injected into the cerebral ventricles 24 hours after or before infection, respectively, for a duration of 7 days. Additionally, all rats received antibiotic subcutaneous treatment starting 24 hours after infection for 7 days. Brainstem auditory evoked potentials were recorded 24 hours before and 24 hours after infection and after 7 days of treatment with brain-derived neurotrophic factor or placebo and antibiotics, respectively, to determine hearing threshold. Our results showed that the hearing thresholds of animals in each group increased significantly 24 hours after infection compared with the results recorded 24 hours before infection (P < .01). After 7 days of treatment with brain-derived neurotrophic factor, brainstem auditory evoked potential responses recurred in 16 ears when stimulated at 75 dB hearing level in groups 1 and 3. Their hearing thresholds significantly decreased compared with the control group 2 (P < .05) and group 4 (P < .01). However, 13 of 14 ears absent brainstem auditory evoked potential responses could still not be identified at 75 dB hearing level in control groups 2 and 4. The improvement of the hearing thresholds in group 3 (treated before infection) was greater than that of group 1 (treated after infection) (P < .05), but there was no significant difference found between the control groups before and after infection (P > .05). Our study supports the hypothesis that the administration of exogenous brain-derived neurotrophic factor can be effective in preventing or treating hearing loss following bacterial meningitis.
基金：
Education Committee of Yunnan Province (No. 0112599).
第一作者机构：[1]Department of Pediatric Neurology,the First People’s Hospital of Yunnan Province, Kunming, Affiliated Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China[*1]Department of Pediatrics, The First People’s Hospital of Yunnan Province, Kunming, 650032, China
通讯作者：
通讯机构：[1]Department of Pediatric Neurology,the First People’s Hospital of Yunnan Province, Kunming, Affiliated Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China[*1]Department of Pediatrics, The First People’s Hospital of Yunnan Province, Kunming, 650032, China
推荐引用方式(GB/T 7714)：
Ling Li,Quan-Xiang Shui,Xianzhen Li.Neuroprotective effects of brain-derived neurotrophic factor (BDNF) on hearing in experimental pneumococcal meningitis[J].JOURNAL OF CHILD NEUROLOGY.2005,20(1):51-56.doi:10.1177/08830738050200010801.
APA：
Ling Li,Quan-Xiang Shui&Xianzhen Li.(2005).Neuroprotective effects of brain-derived neurotrophic factor (BDNF) on hearing in experimental pneumococcal meningitis.JOURNAL OF CHILD NEUROLOGY,20,(1)
MLA：
Ling Li,et al."Neuroprotective effects of brain-derived neurotrophic factor (BDNF) on hearing in experimental pneumococcal meningitis".JOURNAL OF CHILD NEUROLOGY 20..1(2005):51-56
